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PMID: 17344413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast.

Genes & development ·Vol. 21 ·No. 5 ·2007-03-01 ·Pages 519-24

Ito-Harashima S, Kuroha K, Tatematsu T, Inada T

Abstract

mRNA surveillance system represses the expression of nonstop mRNA by rapid mRNA degradation and translation repression. Here we show that the level of protein product of nonstop mRNA containing a poly(A) tail was reduced 100-fold, and this reduction was due to rapid mRNA degradation, translation repression, and protein destabilization, at least in part, by the proteasome. Insertion of a poly(A) tract upstream of a termination codon resulted in translation repression and protein destabilization, but not rapid mRNA decay. We propose that translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization.

MeSH Terms

Codon, Terminator Gene Expression Regulation, Fungal Genes, Reporter Poly A/genetics,metabolism Polylysine/genetics Proteasome Endopeptidase Complex/metabolism Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/genetics,metabolism Ribosomes/metabolism Saccharomyces cerevisiae/genetics,metabolism

Chemicals

Codon, Terminator RNA, Messenger Poly A Polylysine Proteasome Endopeptidase Complex

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ito-Harashima Sayoko

Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan

Kuroha Kazushige

Tatematsu Tsuyako

Inada Toshifumi

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Article Info

Journal

Genes & development

Abbr.

Genes Dev

ISSN

0890-9369

DOI

10.1101/gad.1490207

Published

2007-03-01

Pages

519-24

Language

English

Region

United States

NLM ID

8711660

PMCID

PMC1820893

Subset

External Links

PubMed source DOI: 10.1101/gad.1490207 PMC Full Text View journal details

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Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast.

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