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PMID: 1741253 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro definition of the yeast RNA polymerase I promoter.

Nucleic acids research ·Vol. 20 ·No. 2 ·1992-01-25 ·Pages 279-85

Choe SY, Schultz MC, Reeder RH

Abstract

The structure of the ribosomal gene promoter from Saccharomyces cerevisiae has been analyzed in a whole cell in vitro extract. The promoter contains at least two essential domains, an upstream domain located at the 5' boundary near position -150 and a core promoter domain around the site of transcription initiation at +1. The upstream domain augments transcription in vitro but is not absolutely required. Maintenance of correct spacing between the two domains is critical. The in vitro analysis agrees well with prior in vivo analysis and it appears that the yeast promoter has a structure very similar to that of vertebrate ribosomal gene promoters.

MeSH Terms
Base Sequence Genes, Fungal/genetics Molecular Sequence Data Mutation/genetics Promoter Regions, Genetic/genetics RNA Polymerase I/genetics,metabolism RNA, Ribosomal/genetics Saccharomyces cerevisiae/genetics
Chemicals
RNA, Ribosomal RNA Polymerase I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Choe S Y
Hutchinson Cancer Research Center, Seattle, WA 98104.
Schultz M C
Reeder R H
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.2.279
Published
1992-01-25
Pages
279-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310367
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.2.279 PMC Full Text View journal details
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