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PMID: 17449810 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

MONODEHYROASCORBATE REDUCTASE4 is required for seed storage oil hydrolysis and postgerminative growth in Arabidopsis.

The Plant cell ·Vol. 19 ·No. 4 ·2007-04-00 ·Pages 1376-87

Eastmond PJ

Abstract

Hydrogen peroxide is a major by-product of peroxisomal metabolism and has the potential to cause critical oxidative damage. In all eukaryotes, catalase is thought to be instrumental in removing this H(2)O(2). However, plants also contain a peroxisomal membrane-associated ascorbate-dependent electron transfer system, using ascorbate peroxidase and monodehydroascorbate reductase (MDAR). Here, I report that the conditional seedling-lethal sugar-dependent2 mutant of Arabidopsis thaliana is deficient in the peroxisomal membrane isoform of MDAR (MDAR4). Following germination, Arabidopsis seeds rely on storage oil breakdown to supply carbon skeletons and energy for early seedling growth, and massive amounts of H(2)O(2) are generated within the peroxisome as a by-product of fatty acid beta-oxidation. My data suggest that the membrane-bound MDAR4 component of the ascorbate-dependent electron transfer system is necessary to detoxify H(2)O(2), which escapes the peroxisome. This function appears to be critical to protect oil bodies that are in close proximity to peroxisomes from incurring oxidative damage, which otherwise inactivates the triacylglycerol lipase SUGAR-DEPENDENT1 and cuts off the supply of carbon for seedling establishment.

MeSH Terms
Arabidopsis/growth & development,ultrastructure Arabidopsis Proteins/metabolism Germination Hydrolysis Molecular Sequence Data NADH, NADPH Oxidoreductases/metabolism Organelles/enzymology,ultrastructure Plant Oils/metabolism Seeds/physiology
Chemicals
Arabidopsis Proteins Plant Oils NADH, NADPH Oxidoreductases monodehydroascorbate reductase 4, Arabidopsis monodehydroascorbate reductase (NADH)
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Eastmond Peter J
Warwick HRI, University of Warwick, Wellesbourne, Warwickshire, CV35 9EF, UK. [email protected]
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.043992
Email
[email protected]
Published
2007-04-00
Epub
2007-00-20
Pages
1376-87
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1913749
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BBS/E/H/00PE0274 · United Kingdom
Biotechnology and Biological Sciences Research Council · JF16985 · United Kingdom
Databases
GENBANK
AY039980
External Links
PubMed source DOI: 10.1105/tpc.106.043992 PMC Full Text View journal details
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