Abstract
A microsomal fraction rich in Na+, K+-ATPase (sodium-plus-potassium ion-dependent adenosine triphosphatase) and the corresponding K+-dependent p-nitrophenyl phosphatase from the rectal salt gland of the spiny dogfish was solubilized by treatment with deoxycholate at high ionic strength. On gel filtration through Sepharose 6B, the ATPase apoenzyme could be separated, in apparently soluble form, from the tissue-fraction phospholipids and was almost free of enzymic activity (2% of the p-nitrophenyl phosphatase activity and 0.2% of the ATPase activity being recovered). On mixing the apoenzyme with an activator consisting of cooked ox brain, a large proportion of the original enzymic activity was obtained. Specific activities of the re-activated enzyme were somewhat higher than in the material before gel filtration: values of 1300-1450 mumol and 250-290 mumol/h per mg of protein were obtained for the hydrolysis of ATP and of p-nitrophenyl phosphate respectively. The activity was inhibitible by ouabain.
MeSH Terms
4-Nitrophenylphosphatase/metabolism
Adenosine Triphosphatases/antagonists & inhibitors,isolation & purification
Adenosine Triphosphate/metabolism
Animals
Apoenzymes
Cattle
Deoxycholic Acid
Dogfish
Enzyme Activation
Hydrolysis
Lipids/pharmacology
Ouabain/pharmacology
Phospholipids/isolation & purification
Potassium
Salt Gland/enzymology
Sodium
Chemicals
Apoenzymes
Lipids
Phospholipids
Deoxycholic Acid
Ouabain
Adenosine Triphosphate
Sodium
4-Nitrophenylphosphatase
Adenosine Triphosphatases
Potassium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ottolenghi P
References (11)
11 references, click to expand
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