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PMID: 1750 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of an unsaturated fatty acid-requiring mutant of cultured mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 1 ·1976-01-00 ·Pages 24-8

Chang TY, Vagelos PR

Abstract

An unsaturated fatty acid-requiring mutant derived from Chinese hamster ovary (CHO) cells has been isolated and characterized. This mutant grows normally when oleate or other unsaturated fatty acids are supplemented in the growth medium. Unlike the wild-type CHO cells, growth stops when medium is deprived of unsaturated fatty acid. Whole cell pulse experiments with [14C]acetate or [14C]stearate indicate that the mutant is defective in unsaturated fatty acid synthesis. Enzyme assays in vitro show that the enzymatic defect of the mutant is localized to the microsomal stearoyl-CoA desaturase.

MeSH Terms
Acetates/metabolism Cell Division/drug effects Cell Line Cell Membrane/metabolism Cell Separation Fatty Acid Desaturases/metabolism Fatty Acids/biosynthesis Fatty Acids, Unsaturated/metabolism,pharmacology Hot Temperature Microsomes/enzymology Mutation NADPH-Ferrihemoprotein Reductase/metabolism Oleic Acids/metabolism Stearoyl-CoA Desaturase/metabolism
Chemicals
Acetates Fatty Acids Fatty Acids, Unsaturated Oleic Acids Fatty Acid Desaturases Stearoyl-CoA Desaturase NADPH-Ferrihemoprotein Reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chang T Y
Vagelos P R
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.1.24
Published
1976-01-00
Pages
24-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335831
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.1.24 PMC Full Text View journal details
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