Home LiteratureArticle Details
PMID: 1750501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

AIDS-associated Kaposi's sarcoma-derived cells in long-term culture express and synthesize smooth muscle alpha-actin.

The American journal of pathology ·Vol. 139 ·No. 6 ·1991-12-00 ·Pages 1251-8

Weich HA, Salahuddin SZ, Gill P, Nakamura S, Gallo RC, Folkmann J

Abstract

Spindle-shaped cells from Kaposi's sarcoma lesions (AIDS-KS cells) were cultured for long periods in the presence of conditioned medium from activated CD4-positive T cells (HTLV-II infected transformed nonvirus producer) and characterized under in vitro conditions. To investigate a possible vascular origin, AIDS-KS cells were analyzed for the presence of smooth muscle alpha-actin, a differentiation marker for vascular smooth muscle cells. Immunofluorescence studies using a monoclonal antibody for smooth muscle alpha-actin demonstrated positive staining of the AIDS-KS cells (KS-3 and KS-4) but not by endothelial cells or fibroblasts. Northern blot analysis using an oligonucleotide probe unique for human smooth muscle alpha-actin indicated the expression of this gene by AIDS-KS cells. Similar analysis of biopsies from the KS lesion showed that in addition to the staining of smooth muscle cells associated with the blood vessels, the tumor-related spindle cells also stained positively. These cells were also analyzed for the expression of different growth factor genes. The platelet-derived growth factor (PDGF) A-chain gene was expressed at a moderate level. The insulin-like growth factor-1 (IGF-1) and insulin-like growth factor-2 (IGF-2) genes were not overexpressed in relation to control cells. These data suggest that the analyzed AIDS-KS cells may be smooth muscle-like cells and therefore of vascular origin. Based on these results as well as previous reports, we speculate that cells of the immune system may regulate growth of cells in the vascular wall by a novel pathway.

MeSH Terms
Acquired Immunodeficiency Syndrome/complications Actins/genetics,metabolism Gene Expression Growth Substances/genetics Humans Muscle, Smooth/metabolism Platelet-Derived Growth Factor/genetics RNA, Messenger/metabolism Reference Values Sarcoma, Kaposi/etiology,metabolism,pathology Skin/metabolism Time Factors Tumor Cells, Cultured
Chemicals
Actins Growth Substances Platelet-Derived Growth Factor RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Weich H A
Department of Surgery, Children's Hospital, Boston, Massachusetts.
Salahuddin S Z
Gill P
Nakamura S
Gallo R C
Folkmann J
References (31)
31 references, click to expand
  1. Cyclosporin A inhibits smooth muscle proliferation in the vascular response to injury.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2303-6 PMID: 3258426
  2. Expression and developmental control of platelet-derived growth factor A-chain and B-chain/Sis genes in rat aortic smooth muscle cells.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1524-8 PMID: 3278316
  3. Expression of the sis gene by endothelial cells in culture and in vivo.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6772-4 PMID: 6208557
  4. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
    J Cell Biol. 1986 Dec;103(6 Pt 2):2787-96 PMID: 3539945
  5. Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6330-4 PMID: 3476948
  6. Platelet-derived growth factor. Phorbol ester induces the expression of the B-chain but not of the A-chain in HEL cells.
    FEBS Lett. 1987 Mar 9;213(1):89-94 PMID: 3470198
  7. The human osteosarcoma cell line U-2 OS expresses a 3.8 kilobase mRNA which codes for the sequence of the PDGF-B chain.
    FEBS Lett. 1986 Mar 31;198(2):344-8 PMID: 3456904
  8. Transforming gene from human stomach cancers and a noncancerous portion of stomach mucosa.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3997-4001 PMID: 3459165
  9. Rat skeletal myoblasts and arterial smooth muscle cells express the gene for the A chain but not the gene for the B chain (c-sis) of platelet-derived growth factor (PDGF) and produce a PDGF-like protein.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6844-8 PMID: 3462731
  10. The long 3'-untranslated regions of the PDGF-A and -B mRNAs are only distantly related.
    FEBS Lett. 1987 Nov 2;223(2):243-6 PMID: 3666150
  11. In situ labelling of vascular endothelium with fluorescent acetylated low density lipoprotein.
    Histochem J. 1985 Dec;17(12):1309-20 PMID: 3833857
  12. Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing.
    Lab Invest. 1990 Jul;63(1):21-9 PMID: 2197503
  13. Expression of acidic and basic fibroblast growth factors in human and bovine vascular smooth muscle cells.
    Growth Factors. 1990;2(4):313-20 PMID: 2337475
  14. Structure of a human smooth muscle actin gene (aortic type) with a unique intron site.
    Mol Cell Biol. 1984 Jun;4(6):1073-8 PMID: 6330528
  15. Sequence of cDNA encoding human insulin-like growth factor I precursor.
    Nature. 1983 Dec 8-14;306(5943):609-11 PMID: 6358902
  16. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  17. Kaposi's sarcoma: a clinicopathologic study with particular reference to its relationship to the reticuloendothelial system.
    Medicine (Baltimore). 1965 Sep;44(5):419-43 PMID: 5318077
  18. Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation.
    J Clin Invest. 1991 Mar;87(3):847-58 PMID: 1671868
  19. Modulation of alpha smooth muscle actin and desmin expression in perisinusoidal cells of normal and diseased human livers.
    Am J Pathol. 1991 May;138(5):1233-42 PMID: 2024709
  20. Expression of insulin-like growth factor-I and -II genes in human smooth muscle tumours.
    EMBO J. 1988 May;7(5):1379-85 PMID: 3409868
  21. Platelet-derived growth factor gene expression in human atherosclerotic plaques and normal artery wall.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2810-4 PMID: 3282240
  22. Angiogenic properties of Kaposi's sarcoma-derived cells after long-term culture in vitro.
    Science. 1988 Oct 21;242(4877):430-3 PMID: 2459779
  23. Kaposi's sarcoma cells: long-term culture with growth factor from retrovirus-infected CD4+ T cells.
    Science. 1988 Oct 21;242(4877):426-30 PMID: 3262925
  24. AIDS-Kaposi's sarcoma-derived cells express cytokines with autocrine and paracrine growth effects.
    Science. 1989 Jan 13;243(4888):223-6 PMID: 2643161
  25. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family.
    Cell. 1987 Aug 28;50(5):729-37 PMID: 2957062
  26. cDNA sequence of human transforming gene hst and identification of the coding sequence required for transforming activity.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2980-4 PMID: 2953031
  27. Characterization of human marrow stromal cells: role in progenitor cell binding and granulopoiesis.
    Blood. 1989 May 15;73(7):1794-800 PMID: 2469500
  28. Cultured human endothelial cells express platelet-derived growth factor A chain.
    Am J Pathol. 1987 Jan;126(1):7-12 PMID: 2433948
  29. Modulation of actin mRNAs in cultured vascular cells by matrix components and TGF-beta 1.
    In Vitro Cell Dev Biol. 1989 May;25(5):424-34 PMID: 2659578
  30. Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins.
    Lab Invest. 1989 Feb;60(2):275-85 PMID: 2644484
  31. Expression of the HST1 oncogene in human germ cell tumors.
    Biochem Biophys Res Commun. 1988 Sep 30;155(3):1324-9 PMID: 3178812
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1991-12-00
Pages
1251-8
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886449
Subset
IM
Grants
NCI NIH HHS · R01-CA37395 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2