Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases.

Analytical biochemistry ·Vol. 195 ·No. 2 ·1991-06-00 ·Pages 207-13

Gurevich VV, Pokrovskaya ID, Obukhova TA, Zozulya SA

Abstract

A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used. The transcription can be scaled up to at least 6-12 ml, yielding 4.5-18 mg of homogeneous biologically active mRNA.

MeSH Terms

DNA-Directed RNA Polymerases/pharmacology Escherichia coli/enzymology Genetic Engineering RNA, Messenger/chemical synthesis Transcription, Genetic

Chemicals

RNA, Messenger DNA-Directed RNA Polymerases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gurevich V V

Branch of M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Pushchino, Moscow Region.

Pokrovskaya I D

Obukhova T A

Zozulya S A

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(91)90318-n

Published

1991-06-00

Pages

207-13

Language

English

Region

United States

NLM ID

0370535

Subset

External Links

PubMed source DOI: 10.1016/0003-2697(91)90318-n View journal details

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