Home LiteratureArticle Details
PMID: 17517843 Published · ppublish English Journal Article

Experimental phage therapy against Staphylococcus aureus in mice.

Antimicrobial agents and chemotherapy ·Vol. 51 ·No. 8 ·2007-08-00 ·Pages 2765-73

Capparelli R, Parlato M, Borriello G, Salvatore P, Iannelli D

Abstract

The present study describes a bacteriophage (M(Sa)) active against Staphylococcus aureus, including methicillin-resistant staphylococcal strains. When inoculated into mice simultaneously with S. aureus A170 (10(8) CFU/mouse), phage (10(9) PFU) rescued 97% of the mice; when applied to nonlethal (5 x 10(6) CFU/mouse) 10-day infections, the phage also fully cleared the bacteria. The phage M(Sa), delivered inside macrophages by S. aureus, kills the intracellular staphylococci in vivo and in vitro. The phage can also prevent abscess formation and reduce the bacterial load and weight of abscesses. These results suggest a potential use of the phage for the control of both local and systemic human S. aureus infections.

MeSH Terms
Animals Colony Count, Microbial Female Humans Macrophages, Peritoneal/microbiology,virology Methicillin Resistance Mice Mice, Inbred BALB C Staphylococcal Infections/microbiology,therapy Staphylococcus Phages/physiology Staphylococcus aureus/drug effects,genetics,virology
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Capparelli Rosanna
Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università degli Studi di Napoli Federico II, Via Università 133, Portici, Naples, Italy.
Parlato Marianna
Borriello Giorgia
Salvatore Paola
Iannelli Domenico
References (35)
35 references, click to expand
  1. Experimental bacteriophage protection against Staphylococcus aureus abscesses in a rabbit model.
    Antimicrob Agents Chemother. 2005 Mar;49(3):1220-1 PMID: 15728933
  2. Antibacterial resistance worldwide: causes, challenges and responses.
    Nat Med. 2004 Dec;10(12 Suppl):S122-9 PMID: 15577930
  3. Detection and characterization of Streptococcus thermophilus bacteriophages by use of the antireceptor gene sequence.
    Appl Environ Microbiol. 2005 Oct;71(10):6096-103 PMID: 16204526
  4. Immune evasion by staphylococci.
    Nat Rev Microbiol. 2005 Dec;3(12):948-58 PMID: 16322743
  5. An siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infection.
    Nature. 2006 Jan 5;439(7072):89-94 PMID: 16306938
  6. Selection of an Escherichia coli O157:H7 bacteriophage for persistence in the circulatory system of mice infected experimentally.
    Clin Microbiol Infect. 2006 Mar;12(3):248-53 PMID: 16451412
  7. Non-inherited antibiotic resistance.
    Nat Rev Microbiol. 2006 Jul;4(7):556-62 PMID: 16778840
  8. Pharmacodynamics of non-replicating viruses, bacteriocins and lysins.
    Proc Biol Sci. 2006 Nov 7;273(1602):2703-12 PMID: 17015317
  9. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance.
    J Clin Microbiol. 2000 Mar;38(3):1032-5 PMID: 10698991
  10. Phage therapy: the peculiar kinetics of self-replicating pharmaceuticals.
    Clin Pharmacol Ther. 2000 Sep;68(3):225-30 PMID: 11014403
  11. Bacteriophage therapy.
    Antimicrob Agents Chemother. 2001 Mar;45(3):649-59 PMID: 11181338
  12. In vivo efficacy of trovafloxacin against Bacteroides fragilis in mixed infection with either Escherichia coli or a vancomycin-resistant strain of Enterococcus faecium in an established-abscess murine model.
    Antimicrob Agents Chemother. 2001 May;45(5):1394-401 PMID: 11302801
  13. Why we don't get sick: the within-host population dynamics of bacterial infections.
    Science. 2001 May 11;292(5519):1112-5 PMID: 11352067
  14. Multiplex PCR protocol for the diagnosis of staphylococcal infection.
    J Clin Microbiol. 2001 Sep;39(9):3332-8 PMID: 11526172
  15. Bacteriophage therapy rescues mice bacteremic from a clinical isolate of vancomycin-resistant Enterococcus faecium.
    Infect Immun. 2002 Jan;70(1):204-10 PMID: 11748184
  16. Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease.
    Infect Immun. 2002 Feb;70(2):631-41 PMID: 11796592
  17. Killing of Mycobacterium avium and Mycobacterium tuberculosis by a mycobacteriophage delivered by a nonvirulent mycobacterium: a model for phage therapy of intracellular bacterial pathogens.
    J Infect Dis. 2002 Oct 15;186(8):1155-60 PMID: 12355367
  18. Human leukocytes adhere to, penetrate, and respond to Staphylococcus aureus biofilms.
    Infect Immun. 2002 Nov;70(11):6339-45 PMID: 12379713
  19. Dynamics of success and failure in phage and antibiotic therapy in experimental infections.
    BMC Microbiol. 2002 Nov 26;2:35 PMID: 12453306
  20. Experimental protection of mice against lethal Staphylococcus aureus infection by novel bacteriophage phi MR11.
    J Infect Dis. 2003 Feb 15;187(4):613-24 PMID: 12599078
  21. Use of genetically engineered phage to deliver antimicrobial agents to bacteria: an alternative therapy for treatment of bacterial infections.
    Antimicrob Agents Chemother. 2003 Apr;47(4):1301-7 PMID: 12654662
  22. Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus.
    Science. 2003 Nov 28;302(5650):1569-71 PMID: 14645850
  23. Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania.
    Antimicrob Agents Chemother. 2004 Jan;48(1):275-80 PMID: 14693550
  24. Real-time monitoring of intracellular Staphylococcus aureus replication.
    J Bacteriol. 2004 Feb;186(4):1065-77 PMID: 14762001
  25. New dawn for phage therapy.
    Lancet Infect Dis. 2004 Mar;4(3):186 PMID: 14998510
  26. Vincent Fischetti--following phages for life. Interview by Marilynn Larkin.
    Lancet Infect Dis. 2004 Apr;4(4):246-9 PMID: 15050944
  27. Antibiotics: a shot in the arm.
    Nature. 2004 Oct 21;431(7011):892-3 PMID: 15496888
  28. Minimum bacterial density for bacteriophage replication: implications for significance of bacteriophages in natural ecosystems.
    Appl Environ Microbiol. 1985 Jan;49(1):19-23 PMID: 3156556
  29. The control of experimental Escherichia coli diarrhoea in calves by means of bacteriophages.
    J Gen Microbiol. 1987 May;133(5):1111-26 PMID: 3309177
  30. Intraabdominal infection: a review.
    Clin Infect Dis. 1994 Jul;19(1):100-16 PMID: 7948510
  31. Smaller fleas ... ad infinitum: therapeutic bacteriophage redux.
    Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3167-8 PMID: 8622908
  32. Long-circulating bacteriophage as antibacterial agents.
    Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3188-92 PMID: 8622911
  33. Staphylococcus aureus infections.
    N Engl J Med. 1998 Aug 20;339(8):520-32 PMID: 9709046
  34. Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene.
    J Clin Microbiol. 1998 Sep;36(9):2548-53 PMID: 9705390
  35. Evidence of an intracellular reservoir in the nasal mucosa of patients with recurrent Staphylococcus aureus rhinosinusitis.
    J Infect Dis. 2005 Sep 15;192(6):1023-8 PMID: 16107955
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.01513-06
Published
2007-08-00
Epub
2007-00-21
Pages
2765-73
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC1932491
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.01513-06 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2