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PMID: 17526728 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

NuA4-directed chromatin transactions throughout the Saccharomyces cerevisiae genome.

Molecular and cellular biology ·Vol. 27 ·No. 15 ·2007-08-00 ·Pages 5327-35

Durant M, Pugh BF

Abstract

Two of the major histone acetyltransferases in Saccharomyces cerevisiae are NuA4 and SAGA, which acetylate histones H4 and H3, respectively. Acetylated H3 and H4 tails have been implicated in binding bromodomain proteins, including Bdf1. Bdf1 interacts with the general transcription factor TFIID, which might promote preinitiation complex (PIC) assembly. Bdf1 also interacts with the SWR complex (SWR-C). SWR-C is responsible for the deposition of the histone H2A variant H2A.Z. The placement of these interactions into a connected pathway of PIC assembly has not been fully established. Moreover, it is not known how widespread and how variable such a pathway might be on a genomic scale. Here we provide genomic evidence for S. cerevisiae that PIC assembly (TFIID occupancy) and chromatin remodeling (SWR-C and H2A.Z occupancy) are linked in large part to NuA4-directed H4 acetylation and subsequent Bdf1 binding, rather than through SAGA-directed H3 acetylation. Bdf1 and its homolog Bdf2 tend to have distinct locations in the genome. However, the deletion of BDF1 leads to the accumulation of Bdf2 at Bdf1-vacated sites. Thus, while Bdf1 and Bdf2 are at least partially redundant in function, their functions in the genome are geographically distinct.

MeSH Terms
Acetylation Chromatin/genetics,metabolism Cluster Analysis Genes, Fungal Genome, Fungal/genetics Histone Acetyltransferases/metabolism Models, Genetic Promoter Regions, Genetic/genetics Protein Binding Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Chromatin Saccharomyces cerevisiae Proteins Histone Acetyltransferases NuA4 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Durant Melissa
Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Pugh B Franklin
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00468-07
Published
2007-08-00
Epub
2007-00-25
Pages
5327-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1952100
Subset
IM
Grants
NIGMS NIH HHS · R01 GM059055 · United States
NIGMS NIH HHS · GM059055 · United States
Databases
GEO
GSE6707
External Links
PubMed source DOI: 10.1128/MCB.00468-07 PMC Full Text View journal details
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