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PMID: 17526915 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases.

Journal of experimental botany ·Vol. 58 ·No. 10 ·2007-00-00 ·Pages 2503-12

Minic Z, Jamet E, Négroni L, Arsene der Garabedian P, Zivy M, Jouanin L

Abstract

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using nanoHPLC-MS/MS and MALDI-TOF MS. 102 glycoproteins were identified. 94% of these proteins were predicted by bioinformatics to be targeted to the secretory pathway and 87% of them were predicted to be localized in the cell wall or at the plasma membrane. 30% of these proteins belong to glycoside hydrolase (GH) families with some of them possibly involved in the hydrolysis of cell wall polysaccharides. The second major class of identified proteins comprises aspartyl and serine proteases. Other proteins are predicted to be oxido-reductases, contain interacting domains, are potentially involved in signalling or have an unknown function. This is, to our knowledge, the first survey of plant cell wall N-glycosylated proteins.

MeSH Terms
Arabidopsis/enzymology,growth & development Arabidopsis Proteins/classification,metabolism,physiology Cell Wall/enzymology Chromatography, Affinity/methods Chromatography, High Pressure Liquid Computational Biology Concanavalin A/chemistry Glycoside Hydrolases/metabolism,physiology Glycosylation Plant Stems/enzymology,growth & development Proteome Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemicals
Arabidopsis Proteins Proteome Concanavalin A Glycoside Hydrolases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Minic Zoran
Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint-Cyr, F-78026, Versailles Cedex, France.
Jamet Elisabeth
Négroni Luc
Arsene der Garabedian P
Zivy Michel
Jouanin Lise
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
0022-0957
DOI
10.1093/jxb/erm082
Published
2007-00-00
Epub
2007-00-26
Pages
2503-12
Language
English
Region
England
NLM ID
9882906
PMCID
PMC2394711
Subset
IM
External Links
PubMed source DOI: 10.1093/jxb/erm082 PMC Full Text View journal details
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