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PMID: 17553974 Published · ppublish English Comparative Study Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Rapid identification of viridans streptococci by mass spectrometric discrimination.

Journal of clinical microbiology ·Vol. 45 ·No. 8 ·2007-08-00 ·Pages 2392-7

Friedrichs C, Rodloff AC, Chhatwal GS, Schellenberger W, Eschrich K

Abstract

Viridans streptococci (VS) are responsible for several systemic diseases, such as endocarditis, abscesses, and septicemia. Unfortunately, species identification by conventional methods seems to be more difficult than species identification of other groups of bacteria. The aim of the present study was to evaluate the use of cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) for the rapid identification of 10 different species of VS. A total of 99 VS clinical isolates, 10 reference strains, and 20 strains from our in-house culture collection were analyzed by MALDI-TOF-MS. To evaluate the mass-spectrometric discrimination results, all strains were identified in parallel by phenotypic and genotypic methods. MALDI-TOF-MS identified 71 isolates as the mitis group, 23 as the anginosus group, and 5 as Streptococcus salivarius. Comparison of the species identification results obtained by the MALDI-TOF-MS analyses and with the phenotypic/genotypic identification systems showed 100% consistency at the species level. Thus, MALDI-TOF-MS seems to be a rapid and reliable method for the identification of species of VS from clinical samples.

MeSH Terms
Bacterial Typing Techniques Humans Polymerase Chain Reaction Sensitivity and Specificity Sequence Analysis, DNA Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Streptococcal Infections/microbiology Viridans Streptococci/chemistry,classification
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Friedrichs C
Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Liebigstr. 24, D-04105 Leipzig, Germany. [email protected]
Rodloff A C
Chhatwal G S
Schellenberger W
Eschrich K
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.00556-07
Email
[email protected]
Published
2007-08-00
Epub
2007-00-06
Pages
2392-7
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC1951256
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.00556-07 PMC Full Text View journal details
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