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PMID: 17562791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cdc14-regulated midzone assembly controls anaphase B.

The Journal of cell biology ·Vol. 177 ·No. 6 ·2007-06-18 ·Pages 981-93

Khmelinskii A, Lawrence C, Roostalu J, Schiebel E

Abstract

Spindle elongation in anaphase of mitosis is a cell cycle-regulated process that requires coordination between polymerization, cross-linking, and sliding of microtubules (MTs). Proteins that assemble at the spindle midzone may be important for this process. In this study, we show that Ase1 and the separase-Slk19 complex drive midzone assembly in yeast. Whereas the conserved MT-bundling protein Ase1 establishes a midzone, separase-Slk19 is required to focus and center midzone components. An important step leading to spindle midzone assembly is the dephosphorylation of Ase1 by the protein phosphatase Cdc14 at the beginning of anaphase. Failure to dephosphorylate Ase1 delocalizes midzone proteins and delays the second, slower phase of anaphase B. In contrast, in cells expressing nonphosphorylated Ase1, anaphase spindle extension is faster, and spindles frequently break. Cdc14 also controls the separase-Slk19 complex indirectly via the Aurora B kinase. Thus, Cdc14 regulates spindle midzone assembly and function directly through Ase1 and indirectly via the separase-Slk19 complex.

MeSH Terms
Anaphase Cell Cycle Proteins/physiology Endopeptidases/physiology Microtubule-Associated Proteins Multiprotein Complexes Phosphoprotein Phosphatases/metabolism,physiology Phosphorylation Schizosaccharomyces Schizosaccharomyces pombe Proteins/metabolism,physiology Separase Spindle Apparatus
Chemicals
Cell Cycle Proteins Microtubule-Associated Proteins Multiprotein Complexes Schizosaccharomyces pombe Proteins CDC14 protein, S pombe Phosphoprotein Phosphatases Endopeptidases Separase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Khmelinskii Anton
Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany.
Lawrence Clare
Roostalu Johanna
Schiebel Elmar
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200702145
Published
2007-06-18
Epub
2007-00-11
Pages
981-93
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064359
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200702145 PMC Full Text View journal details
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