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PMID: 17599839 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Human SFMBT is a transcriptional repressor protein that selectively binds the N-terminal tail of histone H3.

FEBS letters ·Vol. 581 ·No. 17 ·2007-07-10 ·Pages 3289-96

Wu S, Trievel RC, Rice JC

Abstract

Human SFMBT (hSFMBT) is postulated to be a Polycomb (PcG) protein. Similar to other PcG proteins, we found that hSFMBT displays robust transcriptional repressor activity. In addition, hSFMBT localized to the nucleus where it strongly associates with chromatin by directly and selectively binding the N-terminal tail of histone H3. Importantly, we discovered that the four tandem MBT repeats of hSFMBT were sufficient for nuclear matrix-association, N-terminal tail H3 binding, and required for transcriptional repression. These findings indicate that the tandem MBT repeats form a functional structure required for biological activity of hSFMBT and predict similar properties for other MBT domain-containing proteins.

MeSH Terms
Amino Acid Sequence Cell Nucleus/metabolism Cells, Cultured DNA-Binding Proteins/chemistry,metabolism,physiology HeLa Cells Histones/chemistry,metabolism Humans K562 Cells Models, Biological Molecular Sequence Data Organ Specificity Protein Structure, Tertiary Repressor Proteins/chemistry,metabolism,physiology Sequence Homology, Amino Acid Substrate Specificity Transfection
Chemicals
DNA-Binding Proteins Histones Repressor Proteins SFMBT1 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wu Shumin
University of Southern California Keck School of Medicine, Department of Biochemistry and Molecular Biology, Los Angeles, CA 90033, USA.
Trievel Raymond C
Rice Judd C
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Article Info
Journal
FEBS letters
Abbr.
FEBS Lett
ISSN
0014-5793
DOI
10.1016/j.febslet.2007.06.025
Published
2007-07-10
Epub
2007-00-21
Pages
3289-96
Language
English
Region
England
NLM ID
0155157
PMCID
PMC2045647
Subset
IM
Grants
NIGMS NIH HHS · R01 GM073839 · United States
NIGMS NIH HHS · R01 GM075094 · United States
NIGMS NIH HHS · R01 GM075094-01A2 · United States
NIGMS NIH HHS · GM073839 · United States
External Links
PubMed source DOI: 10.1016/j.febslet.2007.06.025 PMC Full Text View journal details
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