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PMID: 1760809 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of a monoclonal antibody directed against the epidermal growth factor receptor binding site.

Cancer immunology, immunotherapy : CII ·Vol. 34 ·No. 1 ·1991-00-00 ·Pages 37-42

Pellegrini R, Centis F, Martignone S, Mastroianni A, Tagliabue E, Tosi E, Ménard S, Colnaghi MI

Abstract

In this work a new monoclonal antibody (mAb), designated MGR1, which recognizes the epidermal growth factor receptor (EGF-R) binding site, is described. The main characteristic of this mAb is its ability to discriminate between cells that express normal levels of EGF-R from cells with overexpression, the detectability threshold by immunocytochemical tests being 5 x 10(4) receptors/cell of 10 microns diameter. MGR1 was found to inhibit EGF binding on the relevant target cells, and vice versa its binding was inhibited by EGF, which indicated that MGR1 recognizes the EGF receptor binding site. MGR1 exerted an inhibitory effect on both the in vitro and in vivo growth of cells with EGF-R overexpression, but had no effect on cells with a normal expression of the receptor. Tumour growth inhibition in athymic mice was also obtained on already implanted tumours. MGR1 therefore seems to be an adequate reagent for the development of immunotherapeutical approaches suitable for the treatment of tumours with EGF-R overexpression.

MeSH Terms
Animals Antibodies, Monoclonal/immunology,therapeutic use Binding Sites Epidermal Growth Factor/metabolism ErbB Receptors/analysis,immunology Humans Mice Mice, Inbred BALB C Neoplasms, Experimental/therapy
Chemicals
Antibodies, Monoclonal Epidermal Growth Factor ErbB Receptors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Pellegrini R
Division of Experimental Oncology E, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Centis F
Martignone S
Mastroianni A
Tagliabue E
Tosi E
Ménard S
Colnaghi M I
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Article Info
Journal
Cancer immunology, immunotherapy : CII
Abbr.
Cancer Immunol Immunother
ISSN
0340-7004
DOI
10.1007/BF01741322
Published
1991-00-00
Pages
37-42
Language
English
Region
Germany
NLM ID
8605732
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01741322 View journal details
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