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PMID: 17615297 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A novel pathway that coordinates mitotic exit with spindle position.

Molecular biology of the cell ·Vol. 18 ·No. 9 ·2007-09-00 ·Pages 3440-50

Nelson SA, Cooper JA

Abstract

In budding yeast, the spindle position checkpoint (SPC) delays mitotic exit until the mitotic spindle moves into the neck between the mother and bud. This checkpoint works by inhibiting the mitotic exit network (MEN), a signaling cascade initiated and controlled by Tem1, a small GTPase. Tem1 is regulated by a putative guanine exchange factor, Lte1, but the function and regulation of Lte1 remains poorly understood. Here, we identify novel components of the checkpoint that operate upstream of Lte1. We present genetic evidence in agreement with existing biochemical evidence for the molecular mechanism of a pathway that links microtubule-cortex interactions with Lte1 and mitotic exit. Each component of this pathway is required for the spindle position checkpoint to delay mitotic exit until the spindle is positioned correctly.

MeSH Terms
Guanine Nucleotide Exchange Factors/metabolism Microtubules/metabolism Mitosis Models, Biological Mutation/genetics Protein Binding Protein Transport Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism Spindle Apparatus/metabolism Suppression, Genetic
Chemicals
Guanine Nucleotide Exchange Factors LTE1 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nelson Scott A
Department of Cell Biology, Washington University in St. Louis, St. Louis, MO 63110, USA.
Cooper John A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e07-03-0242
Published
2007-09-00
Epub
2007-00-05
Pages
3440-50
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1951770
Subset
IM
Grants
NIGMS NIH HHS · R01 GM047337 · United States
NIGMS NIH HHS · GM 47337 · United States
NIGMS NIH HHS · T32 GM07067 · United States
NIGMS NIH HHS · GM 69895 · United States
NIGMS NIH HHS · R01 GM069895-04 · United States
NIGMS NIH HHS · R01 GM069895 · United States
NIGMS NIH HHS · T32 GM007067 · United States
External Links
PubMed source DOI: 10.1091/mbc.e07-03-0242 PMC Full Text View journal details
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