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PMID: 17626041 Published · ppublish English Journal Article Research Support, N.I.H., Intramural

Cockayne syndrome protein B interacts with and is phosphorylated by c-Abl tyrosine kinase.

Nucleic acids research ·Vol. 35 ·No. 15 ·2007-00-00 ·Pages 4941-51

Imam SZ, Indig FE, Cheng WH, Saxena SP, Stevnsner T, Kufe D, Bohr VA

Abstract

The Cockayne Syndrome group B (CSB) protein plays important roles in transcription, transcription-coupled nucleotide excision repair and base excision DNA repair. c-Abl kinase also plays a role in DNA repair as a regulator/coordinator of the DNA damage response. This study presents evidence that the N-terminal region of CSB interacts with the SH3 domain of c-Abl in vitro and in vivo. In addition, c-Abl kinase phosphorylates CSB at Tyr932. The subcellular localization of CSB to the nucleus and nucleolus is altered after phosphorylation by c-Abl. c-Abl-dependent phosphorylation of CSB increased in cells treated with hydrogen peroxide and decreased in cells pre-treated with STI-571, a c-Abl-specific protein kinase inhibitor. Activation of the c-Abl kinase in response to oxidative damage is not observed in CSB null cells. These results suggest that c-Abl and CSB may regulate each other in a reciprocal manner in response to oxidative stress.

MeSH Terms
Animals Cell Line Cells, Cultured DNA Helicases/analysis,chemistry,metabolism DNA Repair Enzymes/analysis,chemistry,metabolism Humans Mice Oxidative Stress Phosphorylation Poly-ADP-Ribose Binding Proteins Proto-Oncogene Proteins c-abl/analysis,metabolism Tyrosine/metabolism
Chemicals
Poly-ADP-Ribose Binding Proteins Tyrosine Proto-Oncogene Proteins c-abl DNA Helicases ERCC6 protein, human DNA Repair Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Imam Syed Z
Laboratory of Molecular Gerontology, National Institutes on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
Indig Fred E
Cheng Wen-Hsing
Saxena Satya P
Stevnsner Tinna
Kufe Donald
Bohr Vilhelm A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkm386
Published
2007-00-00
Epub
2007-00-11
Pages
4941-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1976445
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1093/nar/gkm386 PMC Full Text View journal details
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