Home LiteratureArticle Details
PMID: 176472 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity.

Journal of virology ·Vol. 18 ·No. 1 ·1976-04-00 ·Pages 71-9

Berget SM, Mozer TJ, Warner HR

Abstract

The control of activity of the bacteriophage T5-induced 5'-nucleotidase is dependent upon the amount of T5 parental DNA injected into the cell and expressed. When only the first-step transfer DNA is injected and expressed the amount of 5'-nucleotidase activity observed is two to three times the maximum amount observed after normal T5 infection, and inactivation of the enzyme does not occur. Enzyme inactivation occurs only after the remaining DNA is injected, but only limited expression of this DNA is required. The control of the nucleotidase inactivation process is similar to that for the repair of the nicks in parental DNA, and is probably mediated by a class IIa protein.

MeSH Terms
Calcium/pharmacology Cell-Free System Chloramphenicol/pharmacology Colicins/pharmacology DNA, Viral/metabolism Enzyme Induction Escherichia coli/metabolism Mutation Nucleotidases/metabolism Phosphotransferases/metabolism Viral Proteins/biosynthesis
Chemicals
Colicins DNA, Viral Viral Proteins Chloramphenicol Phosphotransferases Nucleotidases Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Berget S M
Mozer T J
Warner H R
References (21)
21 references, click to expand
  1. Protein kinase of bacteriophage T7. 2. Properties, enzyme synthesis in vitro and regulation of enzyme synthesis and activity in vivo.
    Eur J Biochem. 1975 Jun 16;55(1):305-14 PMID: 240695
  2. Arrangement on the chromosome of the known pre-early genes of bacteriophages T5 and BF23.
    J Virol. 1973 Nov;12(5):1191-4 PMID: 4765399
  3. Abortive infection by bacteriophage BF23 due to the colicin Ib factor. I. Genetic studies of nonrestricted and amber mutants of bacteriophage BF23.
    Genetics. 1971 Jul;68(3):323-40 PMID: 4942554
  4. Effect of calcium ions on synthesis of T5-specific proteins.
    J Biol Chem. 1970 Nov 25;245(22):5897-903 PMID: 4921964
  5. Early events after infection of Escherichia coli by bacteriophage T5. Induction of a 5'-nucleotidase activity and excretion of free bases.
    J Virol. 1975 Feb;15(2):273-80 PMID: 163355
  6. First-step-transfer deoxyribonucleic acid of bacteriophage T5.
    Bacteriol Rev. 1968 Sep;32(3):227-42 PMID: 4879238
  7. Development of coliphage T5: ultrastructural and biochemical studies.
    J Virol. 1972 Mar;9(3):526-43 PMID: 4259394
  8. Inactivation of the ATP-dependent DNase of Escherichia coli after infection with double-stranded DNA phages.
    J Virol. 1974 Dec;14(6):1611-2 PMID: 4610190
  9. Isolation and partial characterization of bacteriophage T5 mutants deficient in the ability to induce deoxynucleoside monophosphate kinase.
    J Virol. 1974 Jul;14(1):78-85 PMID: 4365325
  10. The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IX. Deoxyribonucleic acid methylase in bacteriophage-infected Escherichia coli.
    J Biol Chem. 1966 May 10;241(9):1985-94 PMID: 5329749
  11. In vivo repair of the single-strand interruptions contained in bacteriophage T5 DNA.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):680-4 PMID: 4522782
  12. Genetic and physiological studies of bacteriophage T5. 2. The relationship between phage DNA synthesis and protein synthesis in T5-infected cells.
    Biochem Biophys Res Commun. 1971 May 21;43(4):735-40 PMID: 4935284
  13. A new deoxyribonuclease activity from bacteria infected with T5 bacteriophage.
    FEBS Lett. 1969 Nov 12;5(3):214-217 PMID: 11947280
  14. In vivo repair of bacteriophage t5 DNA: an assay for viral growth control.
    Virology. 1975 Aug;66(2):393-407 PMID: 1098275
  15. Regulation of bacteriophage T5 development by ColI factors.
    J Virol. 1972 May;9(5):804-12 PMID: 4554465
  16. Invasion by bacteriophage T5. III. Stages revealed by changes in susceptibility of early complexes to abortive infection.
    Virology. 1961 Oct;15:127-35 PMID: 14462207
  17. Protein kinase induction in Escherichia coli by bacteriophage T7.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):586-9 PMID: 4592695
  18. The regulation of transcription in bacteriophage T5-infected Escherichia coli.
    Biochemistry. 1975 Mar 11;14(5):989-97 PMID: 1092331
  19. Patterns of protein synthesis in Escherichia coli infected by amber mutants in the first-step-transfer DNA of T5.
    J Mol Biol. 1970 Feb 28;48(1):133-43 PMID: 4915289
  20. Pre-early proteins of bacteriophage T5: structure and function.
    J Mol Biol. 1971 Dec 28;62(3):551-64 PMID: 5136582
  21. Functions of two genes in the first-step-transfer DNA of bacteriophage T5.
    J Mol Biol. 1969 Aug 28;44(1):173-83 PMID: 4897799
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.18.1.71-79.1976
Published
1976-04-00
Pages
71-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515523
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.18.1.71-79.1976 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2