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PMID: 176473 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Host range temperature-sensitive mutants of herpes simplex virus type 2.

Journal of virology ·Vol. 18 ·No. 1 ·1976-04-00 ·Pages 92-102

Westmoreland D, Rapp F

Abstract

Two small-plaque mutants of herpes simplex virus type 2 (HSV-2) (strain 333), whose growth at 39 C was blocked in certain cell types (cell-dependent temperature sensitivity), were compared compared with parental virus in a number of biological assays. One mutant (no. 69) was found to produce a large number of morphologically normal, but noninfectious, particles; under nonpermissive conditions, these mutant particles were able to interfere with the replication of wild-type HSV-2. The other mutant (no. 74), which is known to belong to a different complementation group, appeared to direct little virus DNA synthesis, even at the permissive temperature. Progeny production and virus DNA synthesis in cells infected by mutant 74 were delayed in comparison with wild-type virus-infected cells. Both mutants were found to be more sensitive to UV irradiation than the parental virus; this was especially marked in the case of mutant 74. Moreover, this mutant was found to have a high transforming efficiency at much lower doses of irradiation than those needed to abolish the cytopathic effect of wildtype HSV-2.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic/radiation effects Culture Techniques DNA, Viral/biosynthesis Defective Viruses/growth & development Humans Mutation Rabbits Radiation Effects Simplexvirus/growth & development,metabolism Temperature Ultraviolet Rays Viral Interference Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Westmoreland D
Rapp F
References (36)
36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.18.1.92-102.1976
Published
1976-04-00
Pages
92-102
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515526
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.18.1.92-102.1976 PMC Full Text View journal details
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