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PMID: 17664335 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

An ACAP1-containing clathrin coat complex for endocytic recycling.

The Journal of cell biology ·Vol. 178 ·No. 3 ·2007-07-30 ·Pages 453-64

Li J, Peters PJ, Bai M, Dai J, Bos E, Kirchhausen T, Kandror KV, Hsu VW

Abstract

Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel clathrin coat complex that is regulated by ARF6 for endocytic recycling in two key physiological settings, stimulation-dependent recycling of integrin that is critical for cell migration and insulin-stimulated recycling of glucose transporter type 4 (Glut4), which is required for glucose homeostasis. These findings not only advance a basic understanding of an early mechanistic step in endocytic recycling but also shed key mechanistic insights into major physiological events for which this transport plays a critical role.

MeSH Terms
3T3-L1 Cells ADP-Ribosylation Factor 6 ADP-Ribosylation Factors/metabolism Amino Acid Sequence Animals Cell Membrane/metabolism,ultrastructure Clathrin/genetics,metabolism Coat Protein Complex I/genetics,metabolism Endocytosis/physiology Endosomes/metabolism,ultrastructure GTPase-Activating Proteins/genetics,metabolism Glucose Transporter Type 4/genetics,metabolism Humans Integrins/metabolism Mice Molecular Sequence Data Multiprotein Complexes Sequence Alignment
Chemicals
ACAP1 protein, human ADP-Ribosylation Factor 6 Clathrin Coat Protein Complex I GTPase-Activating Proteins Glucose Transporter Type 4 Integrins Multiprotein Complexes ADP-Ribosylation Factors ARF6 protein, human Arf6 protein, mouse
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Li Jian
Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, Boston, MA 02115, USA.
Peters Peter J
Bai Ming
Dai Jun
Bos Erik
Kirchhausen Tomas
Kandror Konstantin V
Hsu Victor W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200608033
Published
2007-07-30
Pages
453-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064835
Subset
IM
Grants
NIGMS NIH HHS · GM075252 · United States
NIGMS NIH HHS · R01 GM073016 · United States
NIDDK NIH HHS · DK052057 · United States
NIGMS NIH HHS · R01 GM075252 · United States
NIGMS NIH HHS · GM073016 · United States
NIDDK NIH HHS · R56 DK052057 · United States
NIDDK NIH HHS · R01 DK052057 · United States
External Links
PubMed source DOI: 10.1083/jcb.200608033 PMC Full Text View journal details
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