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PMID: 176659 Published · ppublish English Journal Article

Secretion of macrophage neutral proteinase is enhanced by colchicine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 872-6

Gordon S, Werb Z

Abstract

We have studied the effect of colchicine and related compounds on secretion of enzymes by thioglycollated-elicited mouse peritoneal macrophages in culture. Colchicine stimulated secretion of inducible neutral proteinase activities of elastase (EC 3.4.21.11), collagenase (EC 3.4.24.3), gelatinase (pepsin B; EC 3.4.23.2), and azocaseinase 2- to 6-fold for a period of several days, but inhibited the production and release of lysozyme (mucopeptide N-acetylmuramoylhydrolase; EC 3.2.1.17), a noninducible macrophage secretory product. Parallel changes were observed in cell morphology and secretion after treatment with colchicine, Colcemid, and vinblastine, but not with lumicolchicine, and these effects could be gradually reversed by withdrawal of colchicine. Cytochalasin B also stimulated secretion of elastase 2- to 3-fold but did not influence release of lysozyme. These results demonstrate that tubulin-binding drugs may have opposite effects in macrophages than those usually reported for other experimental systems and also provide evidence for the nonparallel discharge of different macrophage secretion products.

MeSH Terms
Colchicine/pharmacology Cytochalasin B/pharmacology Endopeptidases/metabolism Macrophages/drug effects,enzymology,metabolism Microbial Collagenase/metabolism Pancreatic Elastase/metabolism Peptide Hydrolases/metabolism
Chemicals
Cytochalasin B Endopeptidases Peptide Hydrolases Pancreatic Elastase Microbial Collagenase Colchicine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gordon S
Werb Z
References (22)
22 references, click to expand
  1. Proliferation and colony-forming ability of peritoneal exudate cells in liquid culture.
    J Exp Med. 1975 May 1;141(5):1114-32 PMID: 1092793
  2. Colchicine inhibition of plasma protein release from rat hepatocytes.
    J Cell Biol. 1975 Jul;66(1):42-59 PMID: 1141379
  3. Autophagocytosis: in vitro induction by microtuble poisons.
    Exp Cell Res. 1975 Apr;92(1):11-4 PMID: 1169154
  4. Secretion of a specific collagenase by stimulated macrophages.
    J Exp Med. 1975 Aug 1;142(2):346-60 PMID: 167095
  5. Elastase secretion by stimulated macrophages. Characterization and regulation.
    J Exp Med. 1975 Aug 1;142(2):361-77 PMID: 167096
  6. Collagenase production by human skin fibroblasts.
    Biochem Biophys Res Commun. 1975 May 5;64(1):232-40 PMID: 167734
  7. Cytochalasin B increases collagenase production by cells in vitro.
    Nature. 1975 Sep 18;257(5523):243-4 PMID: 169478
  8. Effects of colchicine on collagenase in cultures of rheumatoid synovium.
    Arthritis Rheum. 1971 Nov-Dec;14(6):669-84 PMID: 4109310
  9. Stimulation of steroid secretion by antimicrotubular agents.
    J Biol Chem. 1973 Apr 25;248(8):2691-8 PMID: 4348917
  10. A specific collagenase from rabbit fibroblasts in monolayer culture.
    Biochem J. 1974 Feb;137(2):373-85 PMID: 4363113
  11. Inhibition of phagocytosis and plasma membrane mobility of the cultivated macrophage by cytochalasin B. Role of subplasmalemmal microfilaments.
    J Cell Biol. 1974 Sep;62(3):647-59 PMID: 4368822
  12. Effects of antimicrotubular agents on the secretion of collagen. A biochemical and morphological study.
    J Cell Biol. 1974 Aug;62(2):390-405 PMID: 4426914
  13. Induction of macrophage plasminogen activator by endotoxin stimulation and phagocytosis: evidence for a two-stage process.
    J Exp Med. 1974 Oct 1;140(4):995-1010 PMID: 4427092
  14. The role of microfilaments and microtubules in cell movement, endocytosis and exocytosis.
    Ciba Found Symp. 1973;14:109-48 PMID: 4591633
  15. Selective release of lysosomal hydrolases from phagocytic cells by cytochalasin B.
    Biochem J. 1973 May;134(1):33-41 PMID: 4737379
  16. Secretion of plasminogen activator by stimulated macrophages.
    J Exp Med. 1974 Apr 1;139(4):834-50 PMID: 4816302
  17. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes.
    J Exp Med. 1974 May 1;139(5):1228-48 PMID: 4825244
  18. Effect of colchicine and vinblastine on the topographical separation of membrane functions.
    J Exp Med. 1972 Jul 1;136(1):1-7 PMID: 5033420
  19. Nucleoside transport in mammalian cells. Inhibition by colchicine.
    Biochemistry. 1972 Jul 4;11(14):2573-8 PMID: 5065219
  20. Ameboid movement induced in cultured macrophages by colchicine or vinblastine.
    Exp Cell Res. 1971 Feb;64(2):419-29 PMID: 5542648
  21. The biochemical events of mitosis. I. Synthesis and properties of colchicine labeled with tritium in its acetyl moiety.
    Biochemistry. 1966 Jul;5(7):2463-8 PMID: 5959471
  22. Colchicine effects on lysosomal enzyme induction and intracellular degradation in the cultivated macrophage.
    J Exp Med. 1975 May 1;141(5):1030-46 PMID: 805204
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.3.872
Published
1976-03-00
Pages
872-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336021
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.3.872 PMC Full Text View journal details
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