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PMID: 17668008 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

High-throughput mapping of origins of replication in human cells.

EMBO reports ·Vol. 8 ·No. 8 ·2007-08-00 ·Pages 770-7

Lucas I, Palakodeti A, Jiang Y, Young DJ, Jiang N, Fernald AA, Le Beau MM

Abstract

Mapping origins of replication has been challenging in higher eukaryotes. We have developed a rapid, genome-wide method to map origins of replication in asynchronous human cells by combining the nascent strand abundance assay with a highly tiled microarray platform, and we validated the technique by two independent assays. We applied this method to analyse the enrichment of nascent DNA in three 50-kb regions containing known origins of replication in the MYC, lamin B2 (LMNB2) and haemoglobin beta (HBB) genes, a 200-kb region containing the rare fragile site, FRAXA, and a 1,075-kb region on chromosome 22; we detected most of the known origins and also 28 new origins. Surprisingly, the 28 new origins were small in size and located predominantly within genes. Our study also showed a strong correlation between origin replication timing and chromatin acetylation.

MeSH Terms
Acetylation Cells, Cultured Chromatin/metabolism Chromatin Immunoprecipitation DNA Replication/genetics Genome, Human/genetics Humans Physical Chromosome Mapping/methods Replication Origin/genetics Reverse Transcriptase Polymerase Chain Reaction
Chemicals
Chromatin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lucas Isabelle
Department of Medicine, Section of Hematology/Oncology, The University of Chicago, Chicago, Illinois 60637, USA.
Palakodeti Aparna
Jiang Yanwen
Young David J
Jiang Nan
Fernald Anthony A
Le Beau Michelle M
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7401026
Published
2007-08-00
Epub
2007-00-13
Pages
770-7
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1978075
Subset
IM
Grants
NCI NIH HHS · R01 CA041644 · United States
NCI NIH HHS · CA41644 · United States
External Links
PubMed source DOI: 10.1038/sj.embor.7401026 PMC Full Text View journal details
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