Abstract
We treated 10 children with X-linked SCID (SCID-X1) using gammaretrovirus-mediated gene transfer. Those with sufficient follow-up were found to have recovered substantial immunity in the absence of any serious adverse events up to 5 years after treatment. To determine the influence of vector integration on lymphoid reconstitution, we compared retroviral integration sites (RISs) from peripheral blood CD3(+) T lymphocytes of 5 patients taken between 9 and 30 months after transplantation with transduced CD34(+) progenitor cells derived from 1 further patient and 1 healthy donor. Integration occurred preferentially in gene regions on either side of transcription start sites, was clustered, and correlated with the expression level in CD34(+) progenitors during transduction. In contrast to those in CD34(+) cells, RISs recovered from engrafted CD3(+) T cells were significantly overrepresented within or near genes encoding proteins with kinase or transferase activity or involved in phosphorus metabolism. Although gross patterns of gene expression were unchanged in transduced cells, the divergence of RIS target frequency between transduced progenitor cells and post-thymic T lymphocytes indicates that vector integration influences cell survival, engraftment, or proliferation.
MeSH Terms
Adult
CD3 Complex
Cell Proliferation
Cell Survival/genetics,immunology
Child
Child, Preschool
Female
Follow-Up Studies
Gammaretrovirus
Genetic Vectors
Graft Survival/genetics,immunology
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells/immunology
Humans
Infant
Male
T-Lymphocytes/immunology
Transduction, Genetic
Transplantation, Autologous
Virus Integration
X-Linked Combined Immunodeficiency Diseases/genetics,immunology,therapy
Authors & Affiliations
25 authors, click to expand affiliations / ORCID
Schwarzwaelder Kerstin
National Center for Tumor Diseases, German Cancer Research Center, Heidelberg, Germany.
Howe Steven J
Schmidt Manfred
Brugman Martijn H
Deichmann Annette
Glimm Hanno
Schmidt Sonja
Prinz Claudia
Wissler Manuela
King Douglas J S
Zhang Fang
Parsley Kathryn L
Gilmour Kimberly C
Sinclair Joanna
Bayford Jinhua
Peraj Rachel
Pike-Overzet Karin
Staal Frank J T
de Ridder Dick
Kinnon Christine
Abel Ulrich
Wagemaker Gerard
Gaspar H Bobby
Thrasher Adrian J
von Kalle Christof
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