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PMID: 17825050 Published · ppublish English Journal Article Research Support, N.I.H., Intramural

Condensin function at centromere chromatin facilitates proper kinetochore tension and ensures correct mitotic segregation of sister chromatids.

Genes to cells : devoted to molecular & cellular mechanisms ·Vol. 12 ·No. 9 ·2007-09-00 ·Pages 1075-90

Yong-Gonzalez V, Wang BD, Butylin P, Ouspenski I, Strunnikov A

Abstract

The condensin complex is essential for sister chromatid segregation in eukaryotic mitosis. Nevertheless, in budding yeast, condensin mutations result in massive mis-segregation of chromosomes containing the nucleolar organizer, while other chromosomes, which also contain condensin binding sites, remain genetically stable. To investigate this phenomenon we analyzed the mechanism of the cell-cycle arrest elicited by condensin mutations. Under restrictive conditions, the majority of condensin-deficient cells arrest in metaphase. This metaphase arrest is mediated by the spindle checkpoint, particularly by the spindle-kinetochore tension-controlling pathway. Inactivation of the spindle checkpoint in condensin mutants resulted in frequent chromosome non-disjunction, eliminating the bias in chromosome mis-segregation towards rDNA-containing chromosomes. The spindle tension defect in condensin-impaired cells is likely mediated by structural defects in centromere chromatin reflected by the partial loss of the centromere histone Cse4p. These findings show that, in addition to its essential role in rDNA segregation, condensin mediates segregation of the whole genome by maintaining the centromere structure in Saccharomyces cerevisiae.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Cell Cycle Proteins/genetics,metabolism Centromere/physiology Chromatin/metabolism DNA, Ribosomal/metabolism DNA-Binding Proteins/genetics,metabolism Kinetochores/physiology Mitosis/physiology Models, Biological Multiprotein Complexes/genetics,metabolism Mutation Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sister Chromatid Exchange/physiology
Chemicals
Cell Cycle Proteins Chromatin DNA, Ribosomal DNA-Binding Proteins Multiprotein Complexes Saccharomyces cerevisiae Proteins condensin complexes Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yong-Gonzalez Vladimir
NIH, NICHD, LGRD, Bethesda, Maryland, USA.
Wang Bi-Dar
Butylin Pavel
Ouspenski Ilia
Strunnikov Alexander
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Article Info
Journal
Genes to cells : devoted to molecular & cellular mechanisms
Abbr.
Genes Cells
ISSN
1356-9597
DOI
10.1111/j.1365-2443.2007.01109.x
Published
2007-09-00
Pages
1075-90
Language
English
Region
England
NLM ID
9607379
PMCID
PMC2674963
Subset
IM
Grants
Intramural NIH HHS · Z01 HD001903-11 · United States
Intramural NIH HHS · Z99 AI999999 · United States
External Links
PubMed source DOI: 10.1111/j.1365-2443.2007.01109.x PMC Full Text View journal details
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