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PMID: 17848513 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

MicroRNA regulation of cyclooxygenase-2 during embryo implantation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 38 ·2007-09-18 ·Pages 15144-9

Chakrabarty A, Tranguch S, Daikoku T, Jensen K, Furneaux H, Dey SK

Abstract

The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. Because microRNAs (miRNAs) have major roles in regulating gene expression, we speculated that they participate in directing the highly regulated spatiotemporally expressed genetic network during implantation. Here, we show that two miRNAs, mmu-miR-101a and mmu-miR-199a*, are spatiotemporally expressed in the mouse uterus during implantation coincident with expression of cyclooxygenase-2, a gene critical for implantation. More interestingly, our in vitro gain- and loss-of-function experiments show that cyclooxygenase-2 expression is posttranscriptionally regulated by these two miRNAs. We report on miRNA-mediated regulation of uterine gene expression in the context of implantation. We believe that many other critical genes related to this process are also regulated by miRNAs. Thus, elucidating the physiological roles of uterine miRNAs will help us better understand the genetic control of implantation, the gateway to a successful pregnancy.

MeSH Terms
Animals Cells, Cultured Cyclooxygenase 2/genetics,metabolism Embryo Implantation/genetics Female Gene Expression Regulation, Developmental HeLa Cells Humans Mice Mice, Inbred Strains MicroRNAs/metabolism Pregnancy Uterus/metabolism
Chemicals
MicroRNAs Cyclooxygenase 2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chakrabarty Anindita
Department of Pediatrics, Vanderbilt University Medical Center, 1161 21st Avenue South, Nashville, TN 37232, USA.
Tranguch Susanne
Daikoku Takiko
Jensen Kevin
Furneaux Henry
Dey Sudhansu K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0705917104
Published
2007-09-18
Epub
2007-00-11
Pages
15144-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1986627
Subset
IM
Grants
NIDA NIH HHS · DA06668 · United States
NICHD NIH HHS · R37 HD012304 · United States
NIDA NIH HHS · F31 DA021062 · United States
NICHD NIH HHS · R37 HD12304 · United States
NIDA NIH HHS · R03 DA022226 · United States
NIDA NIH HHS · DA022226 · United States
NIDA NIH HHS · R01 DA006668 · United States
NIDA NIH HHS · R37 DA006668 · United States
External Links
PubMed source DOI: 10.1073/pnas.0705917104 PMC Full Text View journal details
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