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PMID: 178821 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The requirement for membrane sialic acid in the stimulation of superoxide production during phagocytosis by human polymorphonuclear leukocytes.

The Journal of experimental medicine ·Vol. 143 ·No. 6 ·1976-06-01 ·Pages 1308-16

Tsan M, McIntyre PA

Abstract

The effect of desialylation on phagocytosis of latex particles and oxidative metabolism of human polymorphonuclear leukocytes was studied. Removal of 20% total leukocyte sialic acid by bacterial neuraminidase had no effect on phagocytosis of latex particles and phagocytosis-associated activation of hexose monophosphate shunt in human polymorphonuclear leukocytes. In contrast, desialylation prevented the stimulation of superoxide production either by phagocytosis or by concanavalin A. It is concluded that membrane sialic acid is essential for the stimulation of superoxide production by human polymorphonuclear leukocytes.

MeSH Terms
Cell Membrane/physiology Concanavalin A/pharmacology Cytochrome c Group Glucose/metabolism Humans Neuraminidase Neutrophils/immunology,metabolism,ultrastructure Nitroblue Tetrazolium Oxidation-Reduction Oxygen/biosynthesis Phagocytosis Sialic Acids/physiology Superoxides/biosynthesis
Chemicals
Cytochrome c Group Sialic Acids Concanavalin A Superoxides Nitroblue Tetrazolium Neuraminidase Glucose Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tsan M
McIntyre P A
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23 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.6.1308
Published
1976-06-01
Pages
1308-16
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190211
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.6.1308 PMC Full Text View journal details
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