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PMID: 17925396 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

MCPH1 functions in an H2AX-dependent but MDC1-independent pathway in response to DNA damage.

The Journal of biological chemistry ·Vol. 282 ·No. 48 ·2007-11-30 ·Pages 35416-23

Wood JL, Singh N, Mer G, Chen J

Abstract

Microcephalin (MCPH1) is one of the causative genes for the autosomal recessive disorder, primary microcephaly, characterized by dramatic reduction in brain size and mental retardation. MCPH1 also functions in the DNA damage response, participating in cell cycle checkpoint control. However, how MCPH1 is regulated in the DNA damage response still remains unknown. Here we report that the ability of MCPH1 to localize to the sites of DNA double-strand breaks depends on its C-terminal tandem BRCT domains. Although MCPH1 foci formation depends on H2AX phosphorylation after DNA damage, it can occur independently of MDC1. We also show that MCPH1 binds to a phospho-H2AX peptide in vitro with an affinity similar to that of MDC1, and overexpression of wild type, but not C-BRCT mutants of MCPH1, can interfere with the foci formation of MDC1 and 53BP1. Collectively, our data suggest MCPH1 is recruited to double-strand breaks via its interaction with gammaH2AX, which is mediated by MCPH1 C-terminal BRCT domains. These observations support that MCPH1 acts early in DNA damage responsive pathways.

MeSH Terms
Adaptor Proteins, Signal Transducing Cell Cycle Proteins Cell Line Cell Nucleus/metabolism Cell Separation Cytoskeletal Proteins DNA Damage DNA Repair DNA-Binding Proteins/metabolism Flow Cytometry HeLa Cells Histones/metabolism Humans Kinetics Models, Biological Mutation Nerve Tissue Proteins/metabolism,physiology Nuclear Proteins/metabolism Phosphorylation Trans-Activators/metabolism
Chemicals
Adaptor Proteins, Signal Transducing Cell Cycle Proteins Cytoskeletal Proteins DNA-Binding Proteins H2AX protein, human Histones MCPH1 protein, human MDC1 protein, human Nerve Tissue Proteins Nuclear Proteins Trans-Activators
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wood Jamie L
Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Singh Namit
Mer Georges
Chen Junjie
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26 references, click to expand
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
2007-11-30
Epub
2007-00-09
Pages
35416-23
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2128040
Subset
IM
Grants
NCI NIH HHS · CA109449 · United States
NCI NIH HHS · R01 CA092312 · United States
NCI NIH HHS · R01 CA100109 · United States
NCI NIH HHS · CA092312 · United States
NCI NIH HHS · CA100109 · United States
NCI NIH HHS · R01 CA109449 · United States
NIGMS NIH HHS · F31 GM770802 · United States
NCI NIH HHS · R01 CA109449-04 · United States
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