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PMID: 17932251 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac.

Blood ·Vol. 111 ·No. 7 ·2008-04-01 ·Pages 3435-8

Lux CT, Yoshimoto M, McGrath K, Conway SJ, Palis J, Yoder MC

Abstract

The relative contribution of yolk sac (YS)-derived cells to the circulating definitive hematopoietic progenitor cell (HPC) pool that seeds the fetal liver remains controversial due to the presence of systemic circulation and the onset of hematopoiesis within the embryo proper (EP) before liver seeding. Ncx1-/- embryos fail to initiate a heartbeat on embryonic day (E) 8.25, but continue to develop through E10. We detected normal numbers of primitive erythroid progenitors in Ncx1-/- versus wild type (WT) YS, but primitive erythroblasts did not circulate in the Ncx1-/- EP. While there was no significant difference in the number of definitive HPCs in Ncx1-/- versus WT YS through E9.5, the Ncx1-/- EP was nearly devoid of HPCs. Thus, primitive erythroblasts and essentially all definitive HPCs destined to initially seed the fetal liver after E9.5 are generated in the YS between E7.0-E9.5 and are redistributed into the EP via the systemic circulation.

MeSH Terms

Animals Embryo, Mammalian/cytology,embryology Erythroblasts/cytology,metabolism Hematopoietic Stem Cells/cytology,metabolism Liver/cytology,embryology Mice Mice, Knockout Sodium-Calcium Exchanger/genetics,metabolism Yolk Sac/cytology,embryology

Chemicals

NCX1 protein, mouse Sodium-Calcium Exchanger

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Lux Christopher T

Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis 46202-5254, USA.

Yoshimoto Momoko

McGrath Kathleen

Conway Simon J

Palis James

Yoder Mervin C

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Article Info

Journal

Blood

Abbr.

Blood

ISSN

0006-4971

DOI

10.1182/blood-2007-08-107086

Published

2008-04-01

Epub

2007-00-11

Pages

3435-8

Language

English

Region

United States

NLM ID

7603509

PMCID

PMC2275011

Subset

Grants

NHLBI NIH HHS · R01 HL063169 · United States

NHLBI NIH HHS · HL63169 · United States

External Links

PubMed source DOI: 10.1182/blood-2007-08-107086 PMC Full Text View journal details

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All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac.

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