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PMID: 17933905 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Subcellular localization and functional domain studies of DEFECTIVE KERNEL1 in maize and Arabidopsis suggest a model for aleurone cell fate specification involving CRINKLY4 and SUPERNUMERARY ALEURONE LAYER1.

The Plant cell ·Vol. 19 ·No. 10 ·2007-10-00 ·Pages 3127-45

Tian Q, Olsen L, Sun B, Lid SE, Brown RC, Lemmon BE, Fosnes K, Gruis DF, Opsahl-Sorteberg HG, Otegui MS, Olsen OA

Abstract

DEFECTIVE KERNEL1 (DEK1), which consists of a membrane-spanning region (DEK1-MEM) and a calpain-like Cys proteinase region (DEK1-CALP), is essential for aleurone cell formation at the surface of maize (Zea mays) endosperm. Immunolocalization and FM4-64 dye incubation experiments showed that DEK1 and CRINKLY4 (CR4), a receptor kinase implicated in aleurone cell fate specification, colocalized to plasma membrane and endosomes. SUPERNUMERARY ALEURONE LAYER1 (SAL1), a negative regulator of aleurone cell fate encoding a class E vacuolar sorting protein, colocalized with DEK1 and CR4 in endosomes. Immunogold localization, dual-axis electron tomography, and diffusion of fluorescent dye tracers showed that young aleurone cells established symplastic subdomains through plasmodesmata of larger dimensions than those connecting starchy endosperm cells and that CR4 preferentially associated with plasmodesmata between aleurone cells. Genetic complementation experiments showed that DEK1-CALP failed to restore wild-type phenotypes in maize and Arabidopsis thaliana dek1 mutants, and DEK1-MEM also failed to restore wild-type phenotypes in Arabidopsis dek1-1 mutants. Instead, ectopic expression of DEK1-MEM under the control of the cauliflower mosaic virus 35S promoter gave a dominant negative phenotype. These data suggest a model for aleurone cell fate specification in which DEK1 perceives and/or transmits a positional signal, CR4 promotes the lateral movement of aleurone signaling molecules between aleurone cells, and SAL1 maintains the proper plasma membrane concentration of DEK1 and CR4 proteins via endosome-mediated recycling/degradation.

MeSH Terms
Arabidopsis/genetics,metabolism,ultrastructure Arabidopsis Proteins/genetics,metabolism Calpain/genetics,metabolism Cell Differentiation/genetics,physiology Cell Membrane/metabolism Gene Expression Regulation, Plant Immunoblotting Microscopy, Electron, Scanning Microscopy, Electron, Transmission Models, Biological Plant Proteins/genetics,metabolism Plants, Genetically Modified Protein Kinases/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Zea mays/genetics,growth & development,metabolism,ultrastructure
Chemicals
Arabidopsis Proteins Plant Proteins Protein Kinases Calpain DEK1 protein, Arabidopsis
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Tian Qing
Pioneer Hi-Bred International, A DuPont Business, Johnston, Iowa 50131, USA.
Olsen Lene
Sun Beimeng
Lid Stein Erik
Brown Roy C
Lemmon Betty E
Fosnes Kjetil
Gruis Darren Fred
Opsahl-Sorteberg Hilde-Gunn
Otegui Marisa S
Olsen Odd-Arne
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.048868
Published
2007-10-00
Epub
2007-00-12
Pages
3127-45
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2174714
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.106.048868 PMC Full Text View journal details
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