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PMID: 17967883 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

ICBP90, a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formation.

Molecular and cellular biology ·Vol. 28 ·No. 2 ·2008-01-00 ·Pages 705-17

Karagianni P, Amazit L, Qin J, Wong J

Abstract

Methylation of histone H3 on lysine 9 is critical for diverse biological processes including transcriptional repression, heterochromatin formation, and X inactivation. The biological effects of histone methylation are thought to be mediated by effector proteins that recognize and bind to specific patterns of methylation. Using an unbiased in vitro biochemical approach, we have identified ICBP90, a transcription and cell cycle regulator, as a novel methyl K9 H3-specific binding protein. ICBP90 and its murine homologue Np95 are enriched in pericentric heterochromatin of interphase nuclei, and this localization is dependent on H3K9 methylation. Specific binding of ICBP90 to methyl K9 H3 depends on two functional domains, a PHD (plant homeodomain) finger that defines the binding specificity and an SRA (SET- and RING-associated) domain that promotes binding activity. Furthermore, we present evidence that ICBP90 is required for proper heterochromatin formation in mammalian cells.

MeSH Terms
Amino Acid Motifs CCAAT-Enhancer-Binding Proteins/genetics,metabolism Cell Nucleus/metabolism Chromobox Protein Homolog 5 Chromosomal Proteins, Non-Histone/metabolism Gene Expression Regulation HeLa Cells Heterochromatin/metabolism Histones/genetics,metabolism Humans Interphase Lysine/genetics,metabolism Methylation Protein Binding RNA, Small Interfering/genetics Signal Transduction Ubiquitin-Protein Ligases/genetics,metabolism Ubiquitination
Chemicals
CCAAT-Enhancer-Binding Proteins Chromosomal Proteins, Non-Histone Heterochromatin Histones RNA, Small Interfering Chromobox Protein Homolog 5 UHRF1 protein, human UHRF2 protein, human Ubiquitin-Protein Ligases Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Karagianni Panagiota
Department of Medicine, Harvard Medical School and Massachusetts General Hospital Cancer Center, Building 149, Room 7-213, 13th Street, Charlestown, MA 02129, USA. [email protected]
Amazit Larbi
Qin Jun
Wong Jiemin
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01598-07
Email
[email protected]
Published
2008-01-00
Epub
2007-00-29
Pages
705-17
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2223417
Subset
IM
Grants
NIDDK NIH HHS · R01 DK058679 · United States
NIDDK NIH HHS · DK58679 · United States
External Links
PubMed source DOI: 10.1128/MCB.01598-07 PMC Full Text View journal details
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