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PMID: 17997334 Published · ppublish English Journal Article Review

How sugars convey information on protein conformation in the endoplasmic reticulum.

Seminars in cell & developmental biology ·Vol. 18 ·No. 6 ·2007-12-00 ·Pages 732-42

Caramelo JJ, Parodi AJ

Abstract

The N-glycan-dependent quality control of glycoprotein folding prevents endoplasmic reticulum to Golgi exit of folding intermediates, irreparably misfolded glycoproteins and not completely assembled multimeric complexes. It also enhances folding efficiency by preventing aggregation and facilitating formation of proper disulfide bonds. The control mechanism essentially involves four components, resident lectin-chaperones that recognize monoglucosylated polymannose glycans, a lectin-associated oxidoreductase acting on monoglucosylated glycoproteins, a glucosyltransferase and a glucosidase that creates monoglucosylated epitopes in glycans transferred in protein N-glycosylation or removes the glucose units added by the glucosyltransferase. This last enzyme is the only mechanism component sensing glycoprotein conformations as it creates monoglucosylated glycans exclusively in not properly folded species or in not completely assembled complexes. The purpose of the review is to describe the most significant recent findings on the mechanism of glycoprotein folding and assembly quality control and to discuss the main still unanswered questions.

MeSH Terms
Carbohydrates/physiology Glycoproteins/chemistry,metabolism Glycosylation Lectins Polysaccharides Protein Folding
Chemicals
Carbohydrates Glycoproteins Lectins Polysaccharides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Caramelo Julio J
Laboratory of Glycobiology, Fundación Instituto Leloir, Avda. Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina.
Parodi Armando J
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Article Info
Journal
Seminars in cell & developmental biology
Abbr.
Semin Cell Dev Biol
ISSN
1084-9521
DOI
10.1016/j.semcdb.2007.09.006
Published
2007-12-00
Epub
2007-00-08
Pages
732-42
Language
English
Region
England
NLM ID
9607332
PMCID
PMC2196135
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044500-16 · United States
External Links
PubMed source DOI: 10.1016/j.semcdb.2007.09.006 PMC Full Text View journal details
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