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PMID: 18005755 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Invasive and adherent bacterial pathogens co-Opt host clathrin for infection.

Cell host & microbe ·Vol. 2 ·No. 5 ·2007-11-15 ·Pages 340-51

Veiga E, Guttman JA, Bonazzi M, Boucrot E, Toledo-Arana A, Lin AE, Enninga J, Pizarro-Cerdá J, Finlay BB, Kirchhausen T, Cossart P

Abstract

Infection by the bacterium Listeria monocytogenes depends on host cell clathrin. To determine whether this requirement is widespread, we analyzed infection models using diverse bacteria. We demonstrated that bacteria that enter cells following binding to cellular receptors (termed "zippering" bacteria) invade in a clathrin-dependent manner. In contrast, bacteria that inject effector proteins into host cells in order to gain entry (termed "triggering" bacteria) invade in a clathrin-independent manner. Strikingly, enteropathogenic Escherichia coli (EPEC) required clathrin to form actin-rich pedestals in host cells beneath adhering bacteria, even though this pathogen remains extracellular. Furthermore, clathrin accumulation preceded the actin rearrangements necessary for Listeria entry. These data provide evidence for a clathrin-based entry pathway allowing internalization of large objects (bacteria and ligand-coated beads) and used by "zippering" bacteria as part of a general mechanism to invade host mammalian cells. We also revealed a nonendocytic role for clathrin required for extracellular EPEC infections.

MeSH Terms
Actins/metabolism Animals Bacteria/metabolism,pathogenicity Bacterial Adhesion/physiology Cell Line Clathrin/physiology Dynamins/physiology Endocytosis/physiology Enteropathogenic Escherichia coli/pathogenicity,physiology Escherichia coli Infections/microbiology Humans Listeria monocytogenes/pathogenicity,physiology Listeriosis/microbiology Receptors, Cell Surface/metabolism Virulence
Chemicals
Actins Clathrin Receptors, Cell Surface Dynamins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Veiga Esteban
Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris F-75015, France. [email protected]
Guttman Julian A
Bonazzi Matteo
Boucrot Emmanuel
Toledo-Arana Alejandro
Lin Ann E
Enninga Jost
Pizarro-Cerdá Javier
Finlay B Brett
Kirchhausen Tomas
Cossart Pascale
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Article Info
Journal
Cell host & microbe
Abbr.
Cell Host Microbe
ISSN
1934-6069
DOI
10.1016/j.chom.2007.10.001
Email
[email protected]
Published
2007-11-15
Pages
340-51
Language
English
Region
United States
NLM ID
101302316
PMCID
PMC2803069
Subset
IM
Grants
NIAID NIH HHS · U54 AI057159 · United States
NIAID NIH HHS · U54 AI057159-010008 · United States
PHS HHS · US4 A1057159-05 · United States
Corrections
CommentIn
PMID: 18005749
External Links
PubMed source DOI: 10.1016/j.chom.2007.10.001 PMC Full Text View journal details
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