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PMID: 18007656 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Saccharomyces cerevisiae Rad16 mediates ultraviolet-dependent histone H3 acetylation required for efficient global genome nucleotide-excision repair.

EMBO reports ·Vol. 9 ·No. 1 ·2008-01-00 ·Pages 97-102

Teng Y, Liu H, Gill HW, Yu Y, Waters R, Reed SH

Abstract

In yeast, global genome nucleotide-excision repair (GG-NER) requires a protein complex containing Rad7 and Rad16. Rad16 is a member of the switch/sucrose nonfermentable superfamily, and it is presumed that chromatin remodelling is its primary function during repair. We show that RAD16 is required for ultraviolet-dependent hyperacetylation of histone H3 (Lys 9 and Lys 14) at the MFA2 promoter and throughout the genome. The yeast repressor complex Ssn6-Tup1 represses many genes including MFA2. TUP1 deletion results in constitutive hyperacetylation of histone H3, nucleosome disruption and derepression of gene transcription in Tup1-regulated genes. GG-NER in the MFA2 promoter proceeds more rapidly in tup1Delta alpha-cells compared with wild type, even when transcription is inhibited. We show that elevated histone H3 acetylation levels in the MFA2 promoter in tup1Delta alpha-cells result in Rad7- and Rad16-independent GG-NER, and that Rad16 mediates the ultraviolet-induced acetylation of histone H3, necessary for efficient GG-NER.

MeSH Terms
Acetylation/radiation effects Adenosine Triphosphatases/metabolism DNA Repair/radiation effects DNA-Binding Proteins/metabolism Gene Deletion Gene Expression Regulation, Fungal/radiation effects Genes, Fungal Genome, Fungal/genetics,radiation effects Histones/metabolism Lipoproteins/genetics,metabolism Nuclear Proteins/metabolism Pheromones Pyrimidine Dimers/radiation effects Repressor Proteins/metabolism Saccharomyces cerevisiae/enzymology,genetics,radiation effects Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Analysis, DNA Transcription, Genetic/radiation effects Ultraviolet Rays
Chemicals
DNA-Binding Proteins Histones Lipoproteins MFA2 protein, S cerevisiae Nuclear Proteins Pheromones Pyrimidine Dimers RAD7 protein, S cerevisiae Repressor Proteins Saccharomyces cerevisiae Proteins TUP1 protein, S cerevisiae Adenosine Triphosphatases RAD16 protein, S cerevisiae
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Teng Yumin
Department of Pathology, School of Medicine, Cardiff University, Heath Park, Cardiff, UK.
Liu Hairong
Gill Hefin W
Yu Yachuan
Waters Raymond
Reed Simon H
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7401112
Published
2008-01-00
Epub
2007-00-09
Pages
97-102
Language
English
Region
England
NLM ID
100963049
PMCID
PMC2246617
Subset
IM
Grants
Medical Research Council · G0400113 · United Kingdom
Medical Research Council · G9900118 · United Kingdom
External Links
PubMed source DOI: 10.1038/sj.embor.7401112 PMC Full Text View journal details
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