Home LiteratureArticle Details
PMID: 1801923 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Lipoxygenase metabolites of arachidonic and linoleic acids modulate the adhesion of tumor cells to endothelium via regulation of protein kinase C.

Cell regulation ·Vol. 2 ·No. 12 ·1991-12-00 ·Pages 1045-55

Liu B, Timar J, Howlett J, Diglio CA, Honn KV

Abstract

12(S)-hydroxyeicosatetraenoic acid (12[S]-HETE) and 13(S)-hydroxyoctadecadienoic acid (13[S]-HODE), lipoxygenase metabolites of arachidonic acid and linoleic acid, respectively, previously have been suggested to regulate tumor cell adhesion to endothelium during metastasis. Adhesion of rat Walker carcinosarcoma (W256) cells to a rat endothelial cell monolayer was enhanced after treatment with 12(S)-HETE and this 12(S)-HETE enhanced adhesion was blocked by 13(S)-HODE. Protein kinase inhibitors, staurosporine, calphostin C, and 1-(5-isoquinoline-sulfonyl)-2-methylpiperazine, inhibited the 12(S)-HETE enhanced W256 cell adhesion. Depleting W256 cells of protein kinase C (PKC) with phorbol 12-myristate-13-acetate abolished their ability to respond to 12(S)-HETE. Treatment of W256 cells with 12(S)-HETE induced a 100% increase in membrane-associated PKC activity whereas 13(S)-HODE inhibited the effect of 12(S)-HETE on PKC translocation. High-performance liquid chromatographic analysis revealed that in W256 cells 12-HETE and 13-HODE were two of the major lipoxygenase metabilites of arachidonic acid and linoleic acid, respectively. Therefore, these two metabolites may provide an alternative signaling pathway for the regulation of PKC. Further, these findings suggest that the regulation of tumor cell adhesion to endothelium by 12(S)-HETE and 13(S)-HODE may be a PKC-dependent process.

MeSH Terms
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Animals Arachidonic Acid/metabolism Cell Adhesion/drug effects Down-Regulation Endothelium, Vascular/cytology,drug effects Hydroxyeicosatetraenoic Acids/pharmacology Immunohistochemistry Linoleic Acid Linoleic Acids/metabolism,pharmacology Lipoxygenase/metabolism Protein Kinase C/antagonists & inhibitors,metabolism Tumor Cells, Cultured/drug effects,metabolism,pathology
Chemicals
Hydroxyeicosatetraenoic Acids Linoleic Acids Arachidonic Acid 13-hydroxy-9,11-octadecadienoic acid 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Linoleic Acid Lipoxygenase Protein Kinase C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liu B
Department of Radiation Oncology, Wayne State University, Detroit, Michigan.
Timar J
Howlett J
Diglio C A
Honn K V
References (35)
35 references, click to expand
  1. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  3. Arachidonic acid metabolism of cytosolic fractions of Lewis lung carcinoma cells.
    Adv Prostaglandin Thromboxane Leukot Res. 1991;21B:895-900 PMID: 1825434
  4. Localization of 12-hydroxyeicosatetraenoic acid in endothelial cells.
    J Lipid Res. 1990 Dec;31(12):2265-76 PMID: 2090720
  5. Regulation of 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) binding sites on human epidermal cells by interferon-gamma.
    Exp Cell Res. 1990 Dec;191(2):204-8 PMID: 2175266
  6. Inhibition of invasion of invasive human bladder carcinoma cells by protein kinase C inhibitor staurosporine.
    J Natl Cancer Inst. 1990 Nov 21;82(22):1753-6 PMID: 2231770
  7. Tumor promoter-induced membrane-bound protein kinase C regulates hematogenous metastasis.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):612-6 PMID: 3422445
  8. Sphingosine, an inhibitor of protein kinase C, suppresses the insulin-like effects of growth hormone in rat adipocytes.
    Proc Natl Acad Sci U S A. 1989 Jun;86(12):4705-9 PMID: 2660145
  9. Calphostin C (UCN-1028C), a novel microbial compound, is a highly potent and specific inhibitor of protein kinase C.
    Biochem Biophys Res Commun. 1989 Mar 15;159(2):548-53 PMID: 2467670
  10. Bidirectional control of membrane expression and/or activation of the tumor cell IRGpIIb/IIIa receptor and tumor cell adhesion by lipoxygenase products of arachidonic acid and linoleic acid.
    Cancer Res. 1989 Feb 15;49(4):1029-37 PMID: 2492204
  11. Activation of protein kinase C by lipoxin A and other eicosanoids. Intracellular action of oxygenation products of arachidonic acid.
    Biochem Biophys Res Commun. 1986 Feb 13;134(3):1215-22 PMID: 2418836
  12. Inhibition of phorbol ester-induced phenotypic differentiation of HL-60 cells by 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine, a protein kinase inhibitor.
    Cancer Res. 1986 Feb;46(2):583-7 PMID: 3606750
  13. Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase.
    Biochem Biophys Res Commun. 1986 Mar 13;135(2):397-402 PMID: 3457562
  14. Fatty acyl esters potentiate fatty acid induced activation of protein kinase C.
    Biochem Biophys Res Commun. 1989 May 15;160(3):1110-6 PMID: 2499323
  15. Role of tumor cytoskeleton and membrane glycoprotein IRGpIIb/IIIa in platelet adhesion to tumor cell membrane and tumor cell-induced platelet aggregation.
    Cancer Res. 1988 Jul 1;48(13):3787-800 PMID: 2837325
  16. Role of platelet membrane in enhancement of tumor cell adhesion to endothelial cell extracellular matrix.
    Cancer Res. 1987 Dec 15;47(24 Pt 1):6751-62 PMID: 2824041
  17. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  18. Reversed-phase high-pressure liquid chromatography of arachidonic acid metabolites formed by cyclooxygenase and lipoxygenases.
    Anal Biochem. 1985 Jul;148(1):59-69 PMID: 2994521
  19. Lipoxygenase products regulate IRGpIIb/IIIa receptor mediated adhesion of tumor cells to endothelial cells, subendothelial matrix and fibronectin.
    Proc Soc Exp Biol Med. 1988 Oct;189(1):130-5 PMID: 3141931
  20. Hydroxyeicosatetraenoic acids (HETEs).
    Prog Lipid Res. 1988;27(4):271-323 PMID: 3076240
  21. Interactions between cancer cells and the microvasculature: a rate-regulator for metastasis.
    Clin Exp Metastasis. 1989 Mar-Apr;7(2):127-67 PMID: 2465861
  22. Possible involvement of Ca2+ mobilization and protein kinase C activation in the induction of spontaneous metastasis by mouse mammary adenocarcinoma cells.
    Cancer Res. 1989 May 15;49(10):2597-602 PMID: 2496916
  23. Selective activation of the gamma-subspecies of protein kinase C from bovine cerebellum by arachidonic acid and its lipoxygenase metabolites.
    FEBS Lett. 1989 Jan 30;243(2):177-82 PMID: 2492951
  24. Biology of the protein kinase C family.
    Cancer Metastasis Rev. 1989 Dec;8(3):199-214 PMID: 2697470
  25. The eicosanoids and their biochemical mechanisms of action.
    Biochem J. 1989 Apr 15;259(2):315-24 PMID: 2655580
  26. Distinct cellular and regional localization of immunoreactive protein kinase C in rat brain.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4660-4 PMID: 3299372
  27. Increased 13-hydroxyoctadecadienoic acid content in lipopolysaccharide stimulated macrophages.
    Biochem Biophys Res Commun. 1987 Sep 15;147(2):695-700 PMID: 3632694
  28. Phorbol ester-induced downregulation of protein kinase C potentiates insulin receptor tyrosine autophosphorylation: evidence for a major constitutive role in insulin receptor regulation.
    Biochem Soc Trans. 1990 Jun;18(3):494-5 PMID: 2373247
  29. High-affinity binding and lack of growth-promoting activity of 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) in a human epidermal cell line.
    J Invest Dermatol. 1990 Apr;94(4):446-51 PMID: 2313116
  30. Arachidonic acid and related methyl ester mediate protein kinase C activation in intact platelets through the arachidonate metabolism pathways.
    Biochem Biophys Res Commun. 1990 Jun 29;169(3):933-40 PMID: 2114110
  31. Cadherins: a molecular family important in selective cell-cell adhesion.
    Annu Rev Biochem. 1990;59:237-52 PMID: 2197976
  32. The role of protein kinase C in cell surface signal transduction and tumour promotion.
    Nature. 1984 Apr 19-25;308(5961):693-8 PMID: 6232463
  33. Primary culture of rat cerebral microvascular endothelial cells. Isolation, growth, and characterization.
    Lab Invest. 1982 Jun;46(6):554-63 PMID: 7045518
  34. Metastatic tumor cell attachment and invasion assay utilizing vascular endothelial cell monolayers.
    J Histochem Cytochem. 1982 Mar;30(3):214-20 PMID: 7061823
  35. Substitution of 15-hydroxyeicosatetraenoic acid in the phosphoinositide signaling pathway.
    J Biol Chem. 1991 Apr 25;266(12):7570-7 PMID: 1850411
Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.2.12.1045
Published
1991-12-00
Pages
1045-55
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361904
Subset
IM
Grants
NCI NIH HHS · CA 29997-07 · United States
External Links
PubMed source DOI: 10.1091/mbc.2.12.1045 PMC Full Text
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2