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PMID: 18034358 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of microbial community during biofilm development in an anaerobic wastewater treatment reactor.

Microbial ecology ·Vol. 56 ·No. 1 ·2008-07-00 ·Pages 121-32

Fernández N, Díaz EE, Amils R, Sanz JL

Abstract

The formation, structure, and biodiversity of a multispecies anaerobic biofilm inside an Upflow Anaerobic Sludge Bed (UASB) reactor fed with brewery wastewater was examined using complementary microbial ecology methods such us fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), and cloning. The biofilm development can be roughly divided into three stages: an initial attachment phase (0-36 h) characterized by random adhesion of the cells to the surface; a consolidation phase (from 36 h to 2 weeks) defined by the appearance of microcolonies; and maturation phase (from 2 weeks to 2 months). During the consolidation period, proteobacteria with broad metabolic capabilities, mainly represented by members of alpha-Proteobacteria class (Oleomonas, Azospirillum), predominated. Beta-, gamma-, delta- (both syntrophobacteria and sulfate-reducing bacteria) and epsilon- (Arcobacter sp.) Proteobacteria were also noticeable. Archaea first appeared during the consolidation period. A Methanospirillum-like methanogen was detected after 36 h, and this was followed by the detection of Methanosarcina, after 4 days of biofilm development. The mature biofilm displayed a hill and valley topography with cells embedded in a matrix of exopolymers where the spatial distribution of the microorganisms became well-established. Compared to the earlier phases, the biodiversity had greatly increased. Although alpha-Proteobacteria remained as predominant, members of the phyla Firmicutes, Bacteroidete, and Thermotogae were also detected. Within the domain Archaea, the acetoclastic methanogen Methanosaeta concilii become dominant. This study provides insights on the trophic web and the shifts in population during biofilm development in an UASB reactor.

MeSH Terms
Anaerobiosis Archaea/classification,genetics,growth & development,ultrastructure Bacteria/classification,genetics,growth & development,ultrastructure Biofilms/growth & development Bioreactors Ecosystem Electrophoresis/methods In Situ Hybridization, Fluorescence Microscopy, Confocal Microscopy, Electron, Scanning Molecular Sequence Data Phylogeny Proteobacteria/classification,genetics,growth & development RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Waste Disposal, Fluid/methods Water Purification
Chemicals
RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fernández Nuria
Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.
Díaz Emiliano Enrique
Amils Ricardo
Sanz José L
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Article Info
Journal
Microbial ecology
Abbr.
Microb Ecol
ISSN
0095-3628
DOI
10.1007/s00248-007-9330-2
Published
2008-07-00
Epub
2007-00-23
Pages
121-32
Language
English
Region
United States
NLM ID
7500663
Subset
IM
Databases
GENBANK
AY692039, AY692040, AY692041, AY692042, AY692043, AY692044, AY692045, AY692046, AY692047, AY692048, AY692049, AY692050, AY692051, AY692052, AY692053, AY692054, AY692055, AY692056, AY692057, AY692058, AY692059, AY692060, AY692061, AY692062, AY692063, AY692064, AY692065, AY692066, AY692067, AY692068, AY692069, AY692070, AY692071, AY692072, AY692073, AY692074
External Links
PubMed source DOI: 10.1007/s00248-007-9330-2 View journal details
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