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PMID: 180527 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sodium-stimulated alpha-aminoisobutyric acid transport by membrane vesicles from simian virus-transformed mouse cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 1907-11

Hamilton RT, Nilsen-Hamilton M

Abstract

Uptake of alpha-aminoisobutyric acid, by membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3T3 cells transformed by simian virus 40, is stimulated by sodium chloride. Both in the presence and absence of Na+ uptake is time-dependent and osmotically sensitive. The Na+-stimulated uptake is inhibited by other amino acids. The kinetics of transport of alpha-aminoisobutyric acid are shown to be biphasic both in whole cells and in the membrane vesicles. Only the high affinity system is stimulated by sodium in the membrane vesicles. These results demonstrate that observations made on living cells correlate with observations made on isolated membrane vesicles, and indicate that these membrane vesicles have retained the cellular amino acid transport system functionally intact.

MeSH Terms
Amino Acids/pharmacology Aminoisobutyric Acids/metabolism Biological Transport/drug effects Cations, Monovalent/pharmacology Cell Line Cell Membrane Cell Transformation, Neoplastic Cell-Free System Endoplasmic Reticulum Kinetics Membranes/metabolism Simian virus 40 Sodium/pharmacology
Chemicals
Amino Acids Aminoisobutyric Acids Cations, Monovalent Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hamilton R T
Nilsen-Hamilton M
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.6.1907
Published
1976-06-00
Pages
1907-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430416
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.6.1907 PMC Full Text View journal details
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