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PMID: 18079178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Kinetochore microtubule interaction during S phase in Saccharomyces cerevisiae.

Genes & development ·Vol. 21 ·No. 24 ·2007-12-15 ·Pages 3319-30

Kitamura E, Tanaka K, Kitamura Y, Tanaka TU

Abstract

In the budding yeast Saccharomyces cerevisiae, microtubule-organizing centers called spindle pole bodies (SPBs) are embedded in the nuclear envelope, which remains intact throughout the cell cycle (closed mitosis). Kinetochores are tethered to SPBs by microtubules during most of the cell cycle, including G1 and M phases; however, it has been a topic of debate whether microtubule interaction is constantly maintained or transiently disrupted during chromosome duplication. Here, we show that centromeres are detached from microtubules for 1-2 min and displaced away from a spindle pole in early S phase. These detachment and displacement events are caused by centromere DNA replication, which results in disassembly of kinetochores. Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are subsequently transported poleward by microtubules. Our study gives new insights into kinetochore-microtubule interaction and kinetochore duplication during S phase in a closed mitosis.

MeSH Terms
Centromere Chromosomes, Fungal DNA Replication Kinetochores Microscopy, Fluorescence Microtubules S Phase Saccharomyces cerevisiae/cytology
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kitamura Etsushi
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.
Tanaka Kozo
Kitamura Yoko
Tanaka Tomoyuki U
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.449407
Published
2007-12-15
Pages
3319-30
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC2113032
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1101/gad.449407 PMC Full Text View journal details
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