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PMID: 18094747 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A systems biology approach identifies a R2R3 MYB gene subfamily with distinct and overlapping functions in regulation of aliphatic glucosinolates.

PloS one ·Vol. 2 ·No. 12 ·2007-12-19 ·Pages e1322

Sønderby IE, Hansen BG, Bjarnholt N, Ticconi C, Halkier BA, Kliebenstein DJ

Abstract

Glucosinolates are natural metabolites in the order Brassicales that defend plants against both herbivores and pathogens and can attract specialized insects. Knowledge about the genes controlling glucosinolate regulation is limited. Here, we identify three R2R3 MYB transcription factors regulating aliphatic glucosinolate biosynthesis in Arabidopsis by combining several systems biology tools. MYB28 was identified as a candidate regulator of aliphatic glucosinolates based on its co-localization within a genomic region controlling variation both in aliphatic glucosinolate content (metabolite QTL) and in transcript level for genes involved in the biosynthesis of aliphatic glucosinolates (expression QTL), as well as its co-expression with genes in aliphatic glucosinolate biosynthesis. A phylogenetic analysis with the R2R3 motif of MYB28 showed that it and two homologues, MYB29 and MYB76, were members of an Arabidopsis-specific clade that included three characterized regulators of indole glucosinolates. Over-expression of the individual MYB genes showed that they all had the capacity to increase the production of aliphatic glucosinolates in leaves and seeds and induce gene expression of aliphatic biosynthetic genes within leaves. Analysis of leaves and seeds of single knockout mutants showed that mutants of MYB29 and MYB76 have reductions in only short-chained aliphatic glucosinolates whereas a mutant in MYB28 has reductions in both short- and long-chained aliphatic glucosinolates. Furthermore, analysis of a double knockout in MYB28 and MYB29 identified an emergent property of the system since the absence of aliphatic glucosinolates in these plants could not be predicted by the chemotype of the single knockouts. It seems that these cruciferous-specific MYB regulatory genes have evolved both overlapping and specific regulatory capacities. This provides a unique system within which to study the evolution of MYB regulatory factors and their downstream targets.

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics Chromatography, High Pressure Liquid Chromosomes, Plant Gene Expression Regulation, Plant Genes, Plant Glucosinolates/metabolism Plants, Genetically Modified Quantitative Trait Loci Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Electrospray Ionization Systems Biology Transcription Factors/genetics
Chemicals
Arabidopsis Proteins BOTRYTIS SUSCEPTIBLE1 protein, Arabidopsis Glucosinolates Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sønderby Ida Elken
Plant Biochemistry Laboratory, Department of Plant Biology, Center for Molecular Plant Physiology (PlaCe), Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark.
Hansen Bjarne Gram
Bjarnholt Nanna
Ticconi Carla
Halkier Barbara Ann
Kliebenstein Daniel J
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0001322
Published
2007-12-19
Epub
2007-00-19
Pages
e1322
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2147653
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0001322 PMC Full Text View journal details
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