Lanthanum and some other cation-induced changes in fluidity of synaptosomal membrane studied with nitroxide stearate spin labels.

The Journal of membrane biology ·Vol. 27 ·No. 3 ·1976-00-00 ·Pages 283-95

Uyesaka N, Kamino K, Ogawa M, Inouye A, Machida K

Abstract

Using nitroxide fatty acid spin labels, the effects of some cations such as La3+, Cd2+ and Hg2+ on synaptosomal membranes were studied by observing changes in their ESR spectra. The labels were incorporated almost instantaneously into synaptosomes isolated from rat brain cortex. ESR spectra of the spin-labeled synaptosomes were significantly braodened immediately upon adding La3+, Ce3+, Cd2+ or Hg2+ but hardly affected by Ca2+, Sr2+ and Ba2+. The magnitude of the change in the separation of the outer two peaks in ESR spectra (2T') depends on the number (n) of methylene units between the polar head group and the spin-label (nitroxide) group; that is, it increases with decreasing n. Among these ions, the effect of La3+ was the greatest and appeared to be in parallel with the amount of La3+ bound with the synaptosomes. On the other hand, K+, Rb+ or Li+ causes hardly any significant changes.

MeSH Terms

Animals Cadmium/pharmacology Cerebral Cortex Cerium/pharmacology Electron Spin Resonance Spectroscopy Lanthanum/pharmacology Membranes/drug effects Mercury/pharmacology Metals, Alkali/pharmacology Metals, Alkaline Earth/pharmacology Microsomes/drug effects Myelin Sheath/drug effects Rats Spin Labels Stearates Synaptosomes/drug effects

Chemicals

Metals, Alkali Metals, Alkaline Earth Spin Labels Stearates Cadmium Cerium Lanthanum Mercury

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Uyesaka N

Kamino K

Ogawa M

Inouye A

Machida K

References (24)

24 references, click to expand

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Article Info

Journal

The Journal of membrane biology

Abbr.

J Membr Biol

ISSN

0022-2631

DOI

10.1007/BF01869141

Published

1976-00-00

Pages

283-95

Language

English

Region

United States

NLM ID

0211301

Subset

External Links

PubMed source DOI: 10.1007/BF01869141 View journal details

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Lanthanum and some other cation-induced changes in fluidity of synaptosomal membrane studied with nitroxide stearate spin labels.

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