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PMID: 181750 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Independent regulation of endogenous and exogenous avian RNA tumor virus genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2259-63

Hayward WS, Hanafusa H

Abstract

3H-Labeled complementary DNA specific for the envelope glycoprotein (env) gene of avian leukosis-sarcoma viruses was isolated by selective nucleic acid hybridization techniques, and used to analyze the expression of the endogenous provirus. The endogenous provirus in certain cell types termed chicken helper factor positive (chf+) can synthesize the envelope glycoprotein. Env DNA sequences were present in both chf+ and chf- cells, but env RNA was detectable only in positive cell types. When these cells were infected with the Bryan strain of Rous sarcoma virus (BH-RSV), a defective virus which is deleted in the env gene, the levels of endogenous env RNA remained unchanged, although exogenous BH-RSV specific RNA was synthesized in very high amounts. Thus, the infecting virus did not appear to influence the expression of the endogenous virus. Likewise, the endogenous virus did not influence the exogenous virus expression, since similar amounts of BH-RSV specific RNA were present in all infected cell types, regardless of the level of endogenous virus expression.

MeSH Terms
Alpharetrovirus/metabolism Avian Sarcoma Viruses/metabolism DNA, Viral/metabolism Defective Viruses/metabolism Genes Glycoproteins Helper Viruses/metabolism Nucleic Acid Hybridization RNA, Viral/metabolism Viral Proteins Virus Replication
Chemicals
DNA, Viral Glycoproteins RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hayward W S
Hanafusa H
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2259
Published
1976-07-00
Pages
2259-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430520
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2259 PMC Full Text View journal details
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