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PMID: 18175710 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium).

International journal of systematic and evolutionary microbiology ·Vol. 58 ·No. Pt 1 ·2008-01-00 ·Pages 200-14

Martens M, Dawyndt P, Coopman R, Gillis M, De Vos P, Willems A

Abstract

There is a need for easy, practical, reliable and robust techniques for the identification and classification of bacterial isolates to the species level as alternatives to 16S rRNA gene sequence analysis and DNA-DNA hybridization. Here, we demonstrate that multilocus sequence analysis (MLSA) of housekeeping genes is a valuable alternative technique. An MLSA study of 10 housekeeping genes (atpD, dnaK, gap, glnA, gltA, gyrB, pnp, recA, rpoB and thrC) was performed on 34 representatives of the genus Ensifer. Genetic analysis and comparison with 16S and 23S rRNA gene sequences demonstrated clear species boundaries and a higher discrimination potential for all housekeeping genes. Comparison of housekeeping gene sequence data with DNA-DNA reassociation data revealed good correlation at the intraspecies level, but indicated that housekeeping gene sequencing is superior to DNA-DNA hybridization for the assessment of genetic relatedness between Ensifer species. Our MLSA data, confirmed by DNA-DNA hybridizations, support the suggestion that Ensifer xinjiangensis is a later heterotypic synonym of Ensifer fredii.

MeSH Terms

Bacterial Proteins/genetics Bacterial Typing Techniques DNA, Bacterial/analysis Genes, Bacterial Genes, rRNA Molecular Sequence Data Nucleic Acid Hybridization RNA, Ribosomal, 16S/genetics Rhizobiaceae/classification,genetics Sequence Analysis, DNA Species Specificity

Chemicals

Bacterial Proteins DNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Martens Miet

Laboratorium voor Microbiologie (WE10), Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.

Dawyndt Peter

Coopman Renata

Gillis Monique

De Vos Paul

Willems Anne

Article Info

Journal

International journal of systematic and evolutionary microbiology

Abbr.

Int J Syst Evol Microbiol

ISSN

1466-5026

DOI

10.1099/ijs.0.65392-0

Published

2008-01-00

Pages

200-14

Language

English

Region

England

NLM ID

100899600

Subset

Databases

GENBANK

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PubMed source DOI: 10.1099/ijs.0.65392-0 View journal details

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Advantages of multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer (including former Sinorhizobium).

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