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PMID: 1818509 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A modification of the indirect immunofluorescence test for detection of Ehrlichia phagocytophila antibodies.

Acta veterinaria Scandinavica ·Vol. 32 ·No. 4 ·1991-00-00 ·Pages 499-502

Hardeng F

Abstract

A modified technique for production of antigen and performance of the test is described. A suspension of infected neutrophils was directly applied to multiwell slides. Multichannel pipettes may be used for dilution and application of sera. The modification increases the capacity both by production of the antigen and by performance of the test. This paper also gives a quantitative determination of the antibodies.

MeSH Terms
Animals Antibodies, Bacterial/blood Antigens, Bacterial/immunology Ehrlichia/immunology Ehrlichiosis/immunology,veterinary Female Fluorescent Antibody Technique Reproducibility of Results Sheep Sheep Diseases/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Hardeng F
Department of Large Animal Clinical Sciences, Norwegian College of Veterinary Medicine, Oslo.
References (6)
6 references, click to expand
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    Vet Microbiol. 1989 Dec;21(2):133-8 PMID: 2692278
  2. Use of counter immunoelectrophoresis in detection of antibodies to tickborne fever.
    Res Vet Sci. 1988 Jul;45(1):28-30 PMID: 3222549
  3. Isolation of leukocytes from bovine peripheral blood.
    Proc Soc Exp Biol Med. 1973 Mar;142(3):853-6 PMID: 4692017
  4. Preservation of the infectious agent of tick-borne fever in the frozen state.
    J Comp Pathol. 1966 Oct;76(4):413-6 PMID: 6013233
  5. A complement fixation test for tick-borne fever of sheep.
    Br Vet J. 1971 Sep;127(9):xliv-xlvi PMID: 5165019
  6. Improvement of the teflonized slides used in the immunofluorescent antibody technique.
    Acta Vet Scand. 1986;27(2):296-8 PMID: 3541545
Article Info
Journal
Acta veterinaria Scandinavica
Abbr.
Acta Vet Scand
ISSN
0044-605X
Published
1991-00-00
Pages
499-502
Language
English
Region
England
NLM ID
0370400
PMCID
PMC8127887
Subset
IM
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