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PMID: 18199788 Published · ppublish English Journal Article

Candida nivariensis, an emerging pathogenic fungus with multidrug resistance to antifungal agents.

Journal of clinical microbiology ·Vol. 46 ·No. 3 ·2008-03-00 ·Pages 933-8

Borman AM, Petch R, Linton CJ, Palmer MD, Bridge PD, Johnson EM

Abstract

In 2005, Candida nivariensis, a yeast species genetically related to Candida glabrata, was described following its isolation from three patients in a single Spanish hospital. Between 2005 and 2006, 16 fungal isolates with phenotypic similarities to C. nivariensis were submitted to the United Kingdom Mycology Reference Laboratory for identification. The strains originated from various clinical specimens, including deep, usually sterile sites, from patients at 12 different hospitals in the United Kingdom. PCR amplification and sequencing of the D1D2 and internal transcribed spacer 1 (ITS1) regions of the nuclear ribosomal gene cassette confirmed that these isolates from the United Kingdom are genetically identical to C. nivariensis. Biochemically, C. glabrata and C. nivariensis are distinguished by their differential abilities to assimilate trehalose. However, in contrast to the original published findings, we found that C. glabrata isolates, but not C. nivariensis isolates, are capable of assimilating this substrate. Antifungal susceptibility tests revealed that C. nivariensis isolates are less susceptible than C. glabrata isolates to itraconazole, fluconazole, and voriconazole and to have significantly higher flucytosine MICs than C. glabrata strains. Finally, C. nivariensis could be rapidly distinguished from the other common pathogenic fungus species by pyrosequencing of the ITS2 region. In the light of these data, we believe that C. nivariensis should be regarded as a clinically important emerging pathogenic fungus.

MeSH Terms
Antifungal Agents/pharmacology Azoles/pharmacology Base Sequence Candida/classification,drug effects,genetics,isolation & purification Candidiasis/epidemiology,microbiology Communicable Diseases, Emerging/epidemiology,microbiology DNA, Ribosomal Spacer/analysis,genetics Drug Resistance, Multiple, Fungal Humans Microbial Sensitivity Tests Molecular Sequence Data Mycological Typing Techniques Sequence Analysis, DNA/methods United Kingdom/epidemiology
Chemicals
Antifungal Agents Azoles DNA, Ribosomal Spacer
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Borman Andrew M
Mycology Reference Laboratory, HPA South-West Regional Laboratory, Myrtle Road, Bristol BS2 8EL, United Kingdom. [email protected]
Petch Rebecca
Linton Christopher J
Palmer Michael D
Bridge Paul D
Johnson Elizabeth M
References (20)
20 references, click to expand
  1. Candidemia in allogeneic blood and marrow transplant recipients: evolution of risk factors after the adoption of prophylactic fluconazole.
    J Infect Dis. 2000 Jan;181(1):309-16 PMID: 10608780
  2. Molecular identification of unusual pathogenic yeast isolates by large ribosomal subunit gene sequencing: 2 years of experience at the United kingdom mycology reference laboratory.
    J Clin Microbiol. 2007 Apr;45(4):1152-8 PMID: 17251397
  3. Biogeography of the yeasts of ephemeral flowers and their insects.
    FEMS Yeast Res. 2001 Apr;1(1):1-8 PMID: 12702457
  4. Shifting patterns in the epidemiology of nosocomial Candida infections.
    Chest. 2003 May;123(5 Suppl):500S-3S PMID: 12740235
  5. Infections due to resistant Candida species in patients with cancer who are receiving chemotherapy.
    Clin Infect Dis. 1994 Aug;19 Suppl 1:S49-53 PMID: 7948571
  6. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  7. Evaluation of the AUXACOLOR system, a new method of clinical yeast identification.
    J Clin Pathol. 1995 Sep;48(9):807-9 PMID: 7490311
  8. TreeView: an application to display phylogenetic trees on personal computers.
    Comput Appl Biosci. 1996 Aug;12(4):357-8 PMID: 8902363
  9. Nosocomial Candida. Epidemiology, transmission, and prevention.
    Infect Dis Clin North Am. 1997 Jun;11(2):411-25 PMID: 9187954
  10. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  11. National surveillance of nosocomial blood stream infection due to Candida albicans: frequency of occurrence and antifungal susceptibility in the SCOPE Program.
    Diagn Microbiol Infect Dis. 1998 May;31(1):327-32 PMID: 9597393
  12. Comparison of the API Candida system with the AUXACOLOR system for identification of common yeast pathogens.
    J Clin Microbiol. 1999 Mar;37(3):821-3 PMID: 9986865
  13. Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III.
    J Clin Microbiol. 2005 Jan;43(1):284-92 PMID: 15634984
  14. Invasive Candida infections: the changing epidemiology.
    Oncology (Williston Park). 2004 Dec;18(14 Suppl 13):9-14 PMID: 15682589
  15. Emerging fungal diseases.
    Clin Infect Dis. 2005 Aug 15;41(4):521-6 PMID: 16028162
  16. Phenotypic and molecular characterization of Candida nivariensis sp. nov., a possible new opportunistic fungus.
    J Clin Microbiol. 2005 Aug;43(8):4107-11 PMID: 16081957
  17. PCR protocol for specific identification of Candida nivariensis, a recently described pathogenic yeast.
    J Clin Microbiol. 2005 Dec;43(12):6194-6 PMID: 16333128
  18. Epidemiology of Candida albicans infections and role of non-Candida-albicans yeasts.
    Curr Drug Targets. 2006 Apr;7(4):495-504 PMID: 16611037
  19. Ultra-rapid preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter paper technology - a reusable DNA archiving system.
    Med Mycol. 2006 Aug;44(5):389-98 PMID: 16882605
  20. Antifungal susceptibility testing: practical aspects and current challenges.
    Clin Microbiol Rev. 2001 Oct;14(4):643-58, table of contents PMID: 11585779
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
1098-660X
DOI
10.1128/JCM.02116-07
Email
[email protected]
Published
2008-03-00
Epub
2008-00-16
Pages
933-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC2268332
Subset
IM
Databases
GENBANK
AM745268, AM745269, AM745270, AM745271, AM745272, AM745273, AM745274, AM745275, AM745276, AM745277, AM745278, AM745279, AM745280, AM745281, AM745282, AM745283, AM745284, AM745285, AM745286, AM745287, AM745288, AM745289, AM745290, AM745291, AM745292, AM745293, AM745294, AM745295, AM745296, AM745297, AM745298, AM745299, AM745300, AM745301, AM745302, AM745303, AM745304, AM745305, AM745306, AM745307, AM745308, AM745309, AM745310, AM745311, AM745312, AM745313, AM745314, AM745315, AM745316, AM745317, AM745318, AM745319, AM745320, AM745321, AM745322, AM745323, AM745324, AM745325, AM745326, AM745327, AM745328, AM745329, AM745330, AM745331, AM745332, AM745333, AM745334, AM745335, AM745336, AM745337
External Links
PubMed source DOI: 10.1128/JCM.02116-07 PMC Full Text View journal details
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