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PMID: 18205821 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol.

Environmental microbiology ·Vol. 10 ·No. 3 ·2008-03-00 ·Pages 810-8

de la Torre JR, Walker CB, Ingalls AE, Könneke M, Stahl DA

Abstract

The widespread occurrence and diversity of ammonia oxidizing Archaea suggests their contribution to the nitrogen cycle is of global significance. Their distribution appeared limited to low- and moderate-temperature environments until the recent finding of a diagnostic membrane lipid, crenarchaeol, in terrestrial hot springs. We report here the cultivation of a thermophilic nitrifier ('Candidatus Nitrosocaldus yellowstonii'), an autotrophic crenarchaeote growing up to 74 degrees C by aerobic ammonia oxidation. The major core lipid of this archaeon growing at 72 degrees C is crenarchaeol, providing the first direct evidence for its synthesis by a thermophile. These findings greatly extend the upper temperature limit of nitrification and document that the capacity for ammonia oxidation is broadly distributed among the Crenarchaeota.

MeSH Terms

Ammonia/metabolism Archaea/metabolism Crenarchaeota/genetics,metabolism Glyceryl Ethers/isolation & purification,metabolism Hot Springs Hot Temperature Molecular Sequence Data Oxidation-Reduction Temperature

Chemicals

Glyceryl Ethers crenarchaeol Ammonia

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

de la Torre José R

Department of Civil and Environmental Engineering, University of Washington, Seattle, WA 98195-2700, USA.

Walker Christopher B

Ingalls Anitra E

Könneke Martin

Stahl David A

Article Info

Journal

Environmental microbiology

Abbr.

Environ Microbiol

ISSN

1462-2920

DOI

10.1111/j.1462-2920.2007.01506.x

Published

2008-03-00

Epub

2008-00-19

Pages

810-8

Language

English

Region

England

NLM ID

100883692

Subset

Databases

GENBANK

EU239959, EU239960, EU239961, EU239962, EU239963, EU239964, EU239965, EU239966, EU239967, EU239968, EU239969, EU239970, EU239971, EU239972, EU239973, EU239974, EU239975, EU239976, EU239977, EU239978, EU239979, EU239980, EU239981, EU239982, EU239983, EU239984, EU239985, EU239986, EU239987, EU239988, EU239989, EU239990, EU239991, EU239992, EU239993, EU239994, EU239995, EU239996, EU239997, EU239998, EU239999, EU240000, EU240001

External Links

PubMed source DOI: 10.1111/j.1462-2920.2007.01506.x View journal details

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Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol.

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