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PMID: 1824936 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery.

The EMBO journal ·Vol. 10 ·No. 1 ·1991-01-00 ·Pages 195-206

Carmo-Fonseca M, Tollervey D, Pepperkok R, Barabino SM, Merdes A, Brunner C, Zamore PD, Green MR, Hurt E, Lamond AI

Abstract

The organization of the major snRNP particles in mammalian cell nuclei has been analysed by in situ labelling using snRNA-specific antisense probes made of 2'-OMe RNA. U3 snRNA is exclusively detected in the nucleolus while all the spliceosomal snRNAs are found in the nucleoplasm outside of nucleoli. Surprisingly, U2, U4, U5 and U6 snRNAs are predominantly observed in discrete nucleoplasmic foci. U1 snRNA is also present in foci but in addition is detected widely distributed throughout the nucleoplasm. An anti-peptide antibody specific for the non-snRNP splicing factor U2AF reveals it to have a similar distribution to U1 snRNA. Co-localization studies using confocal fluorescence microscopy prove that U2AF is present in the snRNA-containing foci. Antibody staining also shows the foci to contain snRNP-specific proteins and m3G-cap structures. The presence of major components of the nuclear splicing apparatus in foci suggests that these structures may play a role in pre-mRNA processing.

MeSH Terms
Antisense Elements (Genetics) Base Sequence Cell Nucleus/metabolism,ultrastructure Fluorescent Antibody Technique HeLa Cells/cytology,metabolism Humans Molecular Sequence Data Oligonucleotide Probes RNA Precursors/genetics,metabolism RNA Splicing Ribonucleoproteins/metabolism,ultrastructure Ribonucleoproteins, Small Nuclear
Chemicals
Antisense Elements (Genetics) Oligonucleotide Probes RNA Precursors Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Carmo-Fonseca M
European Molecular Biology Laboratory, Heidelberg, FRG.
Tollervey D
Pepperkok R
Barabino S M
Merdes A
Brunner C
Zamore P D
Green M R
Hurt E
Lamond A I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-01-00
Pages
195-206
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452630
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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