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PMID: 18251428 Published · ppublish English Journal Article Review

Coupling of secondary active transport with a deltamu-H+. .

Journal of bioenergetics and biomembranes ·Vol. 15 ·No. 6 ·1983-12-00 ·Pages 307-19

Kotyk A

Abstract

Most nutrients and ions in bacteria, yeasts, algae, and plants are transported uphill at the expense of a gradient of the electrochemical potential of protons deltamu-H+ (a type of secondary active transport). Diagnosis of such transports rests on the determination of the transmembrane electrical potential difference deltapsi and the difference of pH at the two membrane sides. The behavior of kinetic parameters K(T) (the half-saturation constant) and J(max), (the maximum rate of transport) upon changing driving ion concentrations and electrical potentials may be used to determine the molecular details of the transport reaction. Equilibrium accumulation ratios of driven solutes are expected to be in agreement with the deltapsi and deltapH measured independently, as well as with the Haldane-type expression involving K(T) and J(max). Different stoichiometries of H+/solute, as well as intramembrane effects of pH and deltapsi, may account for some of the observed inconsistencies.

MeSH Terms
Electrochemistry Hydrogen-Ion Concentration Ion Transport/physiology Kinetics Membrane Potentials Models, Biological Proton-Motive Force/physiology Thermodynamics
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kotyk A
Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Praha-Krc, Czechoslovakia.
References (39)
39 references, click to expand
  1. Transport kinetics of 6-deoxy-D-glucose in Candida parapsilosis.
    Folia Microbiol (Praha). 1978;23(1):18-26 PMID: 23984
  2. Kinetics of sulfate uptake by yeast.
    Biochim Biophys Acta. 1979 Feb 20;551(1):197-206 PMID: 34436
  3. Membrane potentials in yeast cells measured by direct and indirect methods.
    Biochim Biophys Acta. 1981 Apr 22;643(1):265-8 PMID: 7016192
  4. Calculation of intracellular pH from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO); application to skeletal muscle of the dog.
    J Clin Invest. 1959 May;38(5):720-9 PMID: 13654506
  5. Energy requirements for maltose transport in yeast.
    Eur J Biochem. 1977 Oct 17;80(1):97-102 PMID: 21792
  6. Coupling in secondary active transport. Activation of transport by co-transport and-or counter-transport with the fluxes of other solutes.
    Biochim Biophys Acta. 1972 Feb 11;255(2):442-61 PMID: 5057926
  7. Transport protein synthesis in non-growing yeast cells.
    Biochim Biophys Acta. 1982 Sep 27;698(3):243-51 PMID: 6753935
  8. The absorption of protons with specific amino acids and carbohydrates by yeast.
    Biochem J. 1973 Aug;134(4):1031-43 PMID: 4587071
  9. On the mechanism of Na+-dependent glucose transport.
    Ann N Y Acad Sci. 1980;339:46-52 PMID: 6249142
  10. Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow.
    Biochemistry. 1979 Aug 21;18(17):3691-7 PMID: 38836
  11. The stoicheiometry of the absorption of protons with phosphate and L-glutamate by yeasts of the genus Saccharomyces.
    Biochem J. 1975 Mar;146(3):705-12 PMID: 238506
  12. Different proton-sugar stoichiometries for the uptake of glucose analogues by Chlorella vulgaris. Evidence for sugar-dependent proton uptake without concomitant sugar uptake by the proton-sugar symport system.
    Biochim Biophys Acta. 1976 Sep 21;448(1):133-42 PMID: 9152
  13. The proton-translocating ATPase of the fungal plasma membrane.
    Biochim Biophys Acta. 1981 Dec 30;639(3-4):197-223 PMID: 6461354
  14. Light-induced changes of the pH gradient and the membrane potential in H. halobium.
    FEBS Lett. 1976 Jun 1;65(2):175-8 PMID: 6333
  15. The determination of the membrane ptoential of Chlorella vulgaris. Evidence for electrogenic sugar transport.
    Eur J Biochem. 1976 Nov 1;70(1):197-204 PMID: 12943
  16. The relationship between ATP and an electrogenic pump in the plasma membrane of Neurospora crassa.
    J Membr Biol. 1973;14(4):305-38 PMID: 4360924
  17. Intracellular pH.
    Physiol Rev. 1981 Apr;61(2):296-434 PMID: 7012859
  18. Intracellular pH of yeast cells measured with fluorescent probes.
    FEBS Lett. 1982 Apr 5;140(1):22-6 PMID: 7084454
  19. Quantitative measurements of membrane potential in Escherichia coli.
    Biochemistry. 1980 Jul 22;19(15):3585-90 PMID: 6996707
  20. Variable H+/substrate stoicheiometries in Rhodotorula gracilis are caused by a pH-dependent protonation of the carrier(s).
    Biochem J. 1982 Nov 15;208(2):459-64 PMID: 6297468
  21. Models for the active transport of cations...the steady-state analysis.
    Mol Cell Biochem. 1977 Mar 21;15(1):27-44 PMID: 865483
  22. Optical probes of membrane potential.
    J Membr Biol. 1976 Jun 30;27(4):317-34 PMID: 787526
  23. Transport of -aminoisobutyric acid in Saccharomyces cerevisiae.
    Biochim Biophys Acta. 1972 Nov 2;288(2):380-9 PMID: 4563235
  24. Performance and conservation of osmotic work by proton-coupled solute porter systems.
    J Bioenerg. 1973 Jan;4(1):63-91 PMID: 4268692
  25. Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiae.
    Biochemistry. 1979 Oct 16;18(21):4487-99 PMID: 40590
  26. Coupling in secondary transport. Effect of electrical potentials on the kinetics of ion linked co-transport.
    Biochim Biophys Acta. 1976 Aug 4;443(1):49-63 PMID: 8129
  27. Concentrative uptake of amino acids by the Ehrlich mouse ascites carcinoma cell.
    J Biol Chem. 1952 Jan;194(1):57-68 PMID: 14927593
  28. Concentration work and energy dissipation in active transport of glycine into carcinoma cells.
    J Biol Chem. 1957 Sep;228(1):97-111 PMID: 13475299
  29. A possible mechanistic role of the membrane potential in proton-sugar cotransport of Chlorella.
    FEBS Lett. 1978 Mar 1;87(1):157-60 PMID: 24552
  30. Active transport of charged substrates by a proton/sugar co-transport system. Amino-sugar uptake in the yeast Rhodotorula gracilis.
    Biochem J. 1981 Feb 15;194(2):433-41 PMID: 6272730
  31. Energy coupling to potassium transport in Streptococcus faecalis. Interplay of ATP and the protonmotive force.
    J Biol Chem. 1980 Jan 25;255(2):433-40 PMID: 6766127
  32. Quantitative analysis of proton-linked transport systems. Glutamate transport in Staphylococcus aureus.
    Biochem J. 1979 Nov 15;184(2):441-9 PMID: 43145
  33. Optical measurement of membrane potential.
    Rev Physiol Biochem Pharmacol. 1978;83:35-88 PMID: 360357
  34. Effect of high substrate concentrations on active transport parameters.
    Biochim Biophys Acta. 1977 Nov 1;470(3):484-91 PMID: 921964
  35. Kinetic analysis of simultaneously occurring proton-sorbose symport and passive sorbose transport in Saccharomyces fragilis.
    Biochim Biophys Acta. 1980 Nov 4;602(2):419-32 PMID: 6252966
  36. Energy coupling in secondary active transport.
    Biochim Biophys Acta. 1980 May 27;604(1):91-126 PMID: 6248113
  37. The driving force for proton(s) metabolites cotransport in bacterial cells.
    FEBS Lett. 1976 Jul 15;66(2):159-63 PMID: 8335
  38. Conversion of biomembrane-produced energy into electric form. I. Submitochondrial particles.
    Biochim Biophys Acta. 1970 Aug 4;216(1):1-12 PMID: 4395700
  39. The electrochemical proton gradient of Saccharomyces. The role of potassium.
    Eur J Biochem. 1982 Apr 1;123(2):447-53 PMID: 6281011
Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00751052
Published
1983-12-00
Pages
307-19
Language
English
Region
United States
NLM ID
7701859
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00751052 View journal details
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