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PMID: 18256282 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 19 ·No. 4 ·2008-04-00 ·Pages 1763-71

Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, Ohya Y

Abstract

Rho1p is an essential small GTPase that plays a key role in the morphogenesis of Saccharomyces cerevisiae. We show here that the activation of Rho1p is regulated by a cyclin-dependent kinase (CDK). Rho1p is activated at the G1/S transition at the incipient-bud sites by the Cln2p (G1 cyclin) and Cdc28p (CDK) complex, in a process mediated by Tus1p, a guanine nucleotide exchange factor for Rho1p. Tus1p interacts physically with Cln2p/Cdc28p and is phosphorylated in a Cln2p/Cdc28p-dependent manner. CDK phosphorylation consensus sites in Tus1p are required for both Cln2p-dependent activation of Rho1p and polarized organization of the actin cytoskeleton. We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition.

MeSH Terms
Actins/metabolism Binding Sites CDC28 Protein Kinase, S cerevisiae/genetics,metabolism Cell Division Cyclin-Dependent Kinases/genetics,metabolism Cyclins/genetics,metabolism Enzyme Activation G1 Phase Genes, Fungal Guanine Nucleotide Exchange Factors/genetics,metabolism Models, Biological Mutation Phosphorylation Recombinant Fusion Proteins/genetics,metabolism S Phase Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism rho GTP-Binding Proteins/genetics,metabolism
Chemicals
Actins CLN2 protein, S cerevisiae Cyclins Guanine Nucleotide Exchange Factors Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins TUS1 protein, S cerevisiae CDC28 Protein Kinase, S cerevisiae Cyclin-Dependent Kinases RHO1 protein, S cerevisiae rho GTP-Binding Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kono Keiko
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan.
Nogami Satoru
Abe Mitsuhiro
Nishizawa Masafumi
Morishita Shinichi
Pellman David
Ohya Yoshikazu
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e07-09-0950
Published
2008-04-00
Epub
2008-00-06
Pages
1763-71
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2291405
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e07-09-0950 PMC Full Text View journal details
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