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PMID: 18263782 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.

Plant physiology ·Vol. 146 ·No. 4 ·2008-04-00 ·Pages 1673-86

Zhou J, Jiao F, Wu Z, Li Y, Wang X, He X, Zhong W, Wu P

Abstract

Previous research has demonstrated that AtPHR1 plays a central role in phosphate (Pi)-starvation signaling in Arabidopsis thaliana. In this work, two OsPHR genes from rice (Oryza sativa) were isolated and designated as OsPHR1 and OsPHR2 based on amino acid sequence homology to AtPHR1. Their functions in Pi signaling in rice were investigated using transgenic plants. Our results showed that both OsPHR1 and OsPHR2 are involved in Pi-starvation signaling pathway by regulation of the expression of Pi-starvation-induced genes, whereas only OsPHR2 overexpression results in the excessive accumulation of Pi in shoots under Pi-sufficient conditions. Under Pi-sufficient conditions, overexpression of OsPHR2 mimics Pi-starvation stress in rice with enhanced root elongation and proliferated root hair growth, suggesting the involvement of OsPHR2 in Pi-dependent root architecture alteration by both systematic and local pathways. In OsPHR2-overexpression plants, some Pi transporters were up-regulated under Pi-sufficient conditions, which correlates with the strongly increased content of Pi. The mechanism behind the OsPHR2 regulated Pi accumulation will provide useful approaches to develop smart plants with high Pi efficiency.

MeSH Terms
Arabidopsis Proteins/genetics,physiology Cloning, Molecular Oryza/genetics,metabolism Phosphates/metabolism Plant Shoots/metabolism Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Subcellular Fractions/metabolism
Chemicals
Arabidopsis Proteins Phosphates Phr2 protein, Arabidopsis
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zhou Jie
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China.
Jiao FangChang
Wu Zhongchang
Li Yiyi
Wang Xuming
He Xiaowei
Zhong Weiqi
Wu Ping
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.107.111443
Published
2008-04-00
Epub
2008-00-08
Pages
1673-86
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2287342
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.107.111443 PMC Full Text View journal details
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