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PMID: 18266466 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1.

PLoS pathogens ·Vol. 4 ·No. 2 ·2008-02-08 ·Pages e21

Salcedo SP, Marchesini MI, Lelouard H, Fugier E, Jolly G, Balor S, Muller A, Lapaque N, Demaria O, Alexopoulou L, Comerci DJ, Ugalde RA, Pierre P, Gorvel JP

Abstract

Brucella is an intracellular pathogen able to persist for long periods of time within the host and establish a chronic disease. We show that soon after Brucella inoculation in intestinal loops, dendritic cells from ileal Peyer's patches become infected and constitute a cell target for this pathogen. In vitro, we found that Brucella replicates within dendritic cells and hinders their functional activation. In addition, we identified a new Brucella protein Btp1, which down-modulates maturation of infected dendritic cells by interfering with the TLR2 signaling pathway. These results show that intracellular Brucella is able to control dendritic cell function, which may have important consequences in the development of chronic brucellosis.

MeSH Terms
Animals Brucella abortus/growth & development,immunology,pathogenicity Brucellosis/immunology,microbiology,pathology Cell Survival Cells, Cultured Cytokines/metabolism Dendritic Cells/immunology,metabolism,microbiology Disease Models, Animal Down-Regulation Host-Pathogen Interactions Ileum/microbiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Peyer's Patches/microbiology Toll-Like Receptor 2/metabolism
Chemicals
Cytokines TLR2 protein, human Toll-Like Receptor 2
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Salcedo Suzana P
Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Faculté de Sciences de Luminy, Marseille, France.
Marchesini María Ines
Lelouard Hugues
Fugier Emilie
Jolly Gilles
Balor Stephanie
Muller Alexandre
Lapaque Nicolas
Demaria Olivier
Alexopoulou Lena
Comerci Diego J
Ugalde Rodolfo A
Pierre Philippe
Gorvel Jean-Pierre
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
DOI
10.1371/journal.ppat.0040021
Published
2008-02-08
Pages
e21
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC2233671
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.ppat.0040021 PMC Full Text View journal details
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