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PMID: 18267075 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector.

Cell ·Vol. 132 ·No. 3 ·2008-02-08 ·Pages 449-62

Caplan JL, Mamillapalli P, Burch-Smith TM, Czymmek K, Dinesh-Kumar SP

Abstract

Plant innate immunity relies on the recognition of pathogen effector molecules by nucleotide-binding-leucine-rich repeat (NB-LRR) immune receptor families. Previously we have shown the N immune receptor, a member of TIR-NB-LRR family, indirectly recognizes the 50 kDa helicase (p50) domain of Tobacco mosaic virus (TMV) through its TIR domain. We have identified an N receptor-interacting protein, NRIP1, that directly interacts with both N's TIR domain and p50. NRIP1 is a functional rhodanese sulfurtransferase and is required for N to provide complete resistance to TMV. Interestingly, NRIP1 that normally localizes to the chloroplasts is recruited to the cytoplasm and nucleus by the p50 effector. As a consequence, NRIP1 interacts with N only in the presence of the p50 effector. Our findings show that a chloroplastic protein is intimately involved in pathogen recognition. We propose that N's activation requires a prerecognition complex containing the p50 effector and NRIP1.

MeSH Terms
Adaptor Proteins, Signal Transducing/analysis,immunology,metabolism Antigens, Viral/immunology Cell Nucleus/chemistry Chloroplasts/chemistry Cytoplasm/chemistry Immunity, Innate Nuclear Proteins/analysis,immunology,metabolism Nuclear Receptor Interacting Protein 1 Plant Diseases/immunology Plant Proteins/analysis,immunology,metabolism Protein Structure, Tertiary Receptors, Virus/analysis,immunology,metabolism Thiosulfate Sulfurtransferase/metabolism Tobacco/immunology,virology Tobacco Mosaic Virus/immunology Two-Hybrid System Techniques
Chemicals
Adaptor Proteins, Signal Transducing Antigens, Viral Nuclear Proteins Nuclear Receptor Interacting Protein 1 Plant Proteins Receptors, Virus Thiosulfate Sulfurtransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Caplan Jeffrey L
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Mamillapalli Padmavathi
Burch-Smith Tessa M
Czymmek Kirk
Dinesh-Kumar S P
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2007.12.031
Published
2008-02-08
Pages
449-62
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2267721
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062625 · United States
NIGMS NIH HHS · R01 GM062625-06A1 · United States
NIGMS NIH HHS · GM62625 · United States
External Links
PubMed source DOI: 10.1016/j.cell.2007.12.031 PMC Full Text View journal details
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