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PMID: 183021 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cells.

Journal of virology ·Vol. 19 ·No. 2 ·1976-08-00 ·Pages 717-31

Purifoy DJ, Powell KL

Abstract

Affinity chromatography on single-stranded and double-stranded DNA-cellulose indicates that 12 proteins previously identified from herpes simplex virus type 2-infected cells, ranging in molecular weight from 28 X 10(3) to 186 X 10(3), bind to DNA-cellulose. The DNA-binding proteins found in infected cells differed in relative binding strengths for denatured DNA-cellulose. The virus specificity of these DNA-binding proteins was further studied by comparison with DNA-binding proteins isolated from mock-infected cells, and by immunoprecipitation of infected-cell DNA-binding proteins with antisera specific for viral antigens. The promise this technique holds for the purification and study of polypeptides involved in virus DNA replication, recombination, or repair is discussed.

MeSH Terms
Cell Line Chromatography, Affinity DNA/metabolism Molecular Weight Nucleic Acid Denaturation Peptides/analysis,metabolism Protein Binding Simplexvirus/analysis,metabolism Viral Proteins/analysis,metabolism
Chemicals
Peptides Viral Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Purifoy D J
Powell K L
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24 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.19.2.717-731.1976
Published
1976-08-00
Pages
717-31
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354905
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.19.2.717-731.1976 PMC Full Text View journal details
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